- Clone
- W22097A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Leu-19, NKH1, NCAM-1
- Isotype
- Rat IgG2a, κ
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Human peripheral blood lymphocytes were stained with anti-human CD16 (clone 3G8) APC and anti-human CD56 (NCAM) (clone W22097A) PE (left) or rat IgG2a, κ PE isotype control (right).
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 343753 | 25 tests | $100.00 | |||
| 343754 | 100 tests | $260.00 | |||
CD56, also known as NCAM (neural cell adhesion molecule), Leu-19, or NKH1, is a single transmembrane glycoprotein. It is a member of the Ig superfamily. The 140 kD isoform is expressed on NK and NKT cells. CD56 is also expressed in the brain (cerebellum and cortex) and at neuromuscular junctions. Certain large granular lymphocyte (LGL) leukemias, small-cell lung carcinomas, neuronal derived tumors, myelomas, and myeloid leukemias also express CD56. CD56 plays a role in homophilic and heterophilic adhesion via binding to itself or heparan sulfate.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mammalian-derived recombinant protein aa 20-718
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
-
Flow cytometry testing showed that clone W22097A can block the binding of clone HCD56 and W22098E on target cells.
Flow cytometry testing showed that clone W22097A does not affect the binding of clone MEM-188, 5.1H11 and W22098A on target cells. - RRID
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AB_3662242 (BioLegend Cat. No. 343753)
AB_3662242 (BioLegend Cat. No. 343754)
Antigen Details
- Structure
- Ig superfamily, single transmembrane or GPI-anchored glycoprotein
- Distribution
-
NK cells, T subset, neural tissue, some LGL and myeloid leukemias
- Function
- Adhesion
- Ligand/Receptor
- Heparan sulfate
- Cell Type
- Leukemia, Neurons, NK cells, NKT cells
- Biology Area
- Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
- Lanier L, et al. 1991. J Immunol. 146:4421-6.
- Hemperly J, et al. 1990. J Mol Neurosci. 2:71-8.
- Cremer H, et al. 1994. Nature. 367:455-9.
- Gene ID
- 4684 View all products for this Gene ID
- UniProt
- View information about CD56 on UniProt.org
Other Formats
View All CD56 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-human CD56 (NCAM) | W22097A | FC |
| PE anti-human CD56 (NCAM) | W22097A | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD56 (NCAM)
Human peripheral blood lymphocytes were stained with anti-hu... -
PE anti-human CD56 (NCAM)
Human peripheral blood lymphocytes were stained with anti-hu...