- Clone
- 2HUB-159 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- VI C-111
- Other Names
- IFN-γRβ, IFNγAF-1
- Isotype
- Hamster IgG
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Human IFN-γ Rβ transfected L929 cells stained with 2HUB-159 PE
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 308504 | 100 tests | $304.00 | |||
IFN-γ R β chain is a 65 kD type I transmembrane protein, also known as accessory factor (AF-1) or IFN-γRII. IFN-γ receptor is composed of an IFN-γ R α chain (CDw119) and a β chain. They are members of the class II cytokine receptor family. The α chain is required for ligand binding and ligand trafficking to the cells and also plays some role in signal transduction. The β chain is critical for delivering IFN-γ mediated pleiotropic signals. IFN-γ R β chain is broadly expressed on a variety of cells at low levels and upregulated on some activated B cells.
Product Details
- Verified Reactivity
- Human
- Reported Reactivity
- African Green, Baboon, Cynomolgus, Rhesus
- Antibody Type
- Monoclonal
- Host Species
- Hamster
- Immunogen
- soluable extracellular domains of the human IFN-γ R β chain
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
- Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
-
Additional reported applications (for the relevant formats) include: Western blotting2,3 and immunoprecipitation2,3. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 308504) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-Armenian hamster IgG second step (Cat. No. 405501), followed by SAv-PE (Cat. No. 405204)). TThe LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 308506).
-
Application References
(PubMed link indicates BioLegend citation) -
- Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
- Bach EA, et al. 1996. Mol. Cell. Biol. 16:3214. (IP WB)
- Rosenzweig SD, et al. 2004. J. Immunol. 173:4000. (IP WB)
- Joseph TD, et al. 2001. J. Biol. Chem. 276:47136.
- Choi JC, et al. 2007. J. Immunol. 178:1598.
- Tjon AS, et al. 2014. J Immunol. 192:5625. PubMed
- Product Citations
-
- RRID
-
AB_314718 (BioLegend Cat. No. 308504)
Antigen Details
- Structure
- Ig superfamily, class II cytokine receptor family, associates with CDw119, 65 kD
- Distribution
-
All cells, except erythrocytes, at low levels
- Function
- Transducing signals
- Ligand/Receptor
- IFN-γ
- Biology Area
- Immunology
- Molecular Family
- Cytokine/Chemokine Receptors
- Antigen References
-
1. Basler C, et al. 2002. Int. Rev. Immunol. 21:305.
2. Brierley M, et al. 2002. J. Interferon Cytokine Res. 22:835. - Gene ID
- 3459 View all products for this Gene ID
- UniProt
- View information about IFN-gamma R beta chain on UniProt.org
Other Formats
View All IFN-γ R β chain Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| PE anti-human IFN-γ R β chain | 2HUB-159 | FC |
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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PE anti-human IFN-γ R β chain
Human IFN-γ Rβ transfected L929 cells stained with 2HUB-159 ...