PE anti-human IL-2 Antibody

Pricing & Availability
Clone
MQ1-17H12 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-2, T cell growth factor (TCGF), Eosinophil differentiation factor (EDF), Killer cell helper factor (KHF), Macrophage-activating factor for cytotoxicity I (MAF-C I), Thymocyte differentiation factor (TDF)
Isotype
Rat IgG2a, κ
MQ1-17H12_PE_IL-2_Antibody_1_102324
PMA/Ionomycin-stimulated human PBMCs were stained with CD3 PE/Cyanine5 and MQ1-17H12 PE
  • MQ1-17H12_PE_IL-2_Antibody_1_102324
    PMA/Ionomycin-stimulated human PBMCs were stained with CD3 PE/Cyanine5 and MQ1-17H12 PE
Compare all formats See PE spectral data
Cat # Size Price Quantity Check Availability
500306 25 tests $98.00
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500307 100 tests $242.00
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Description

IL-2 is a potent lymphoid cell growth factor which exerts its biological activity primarily on T cells, promoting proliferation and maturation. Additionally, IL-2 has been found to stimulate growth and differentiation of B cells, NK cells, LAK cells, monocytes, and oligodendrocytes.

Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
Cat, Chimpanzee, Baboon, Cynomolgus, Rhesus, Sooty Mangabey
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
E. coli - expressed recombinant human IL-2
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

ELISA or ELISPOT Capture2,3: The purified MQ1-17H12 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the Biotin anti-human IL-2 antibody (Cat. No. 517605) as the detecting antibody. The Ultra-LEAF™ purified antibody is suggested for ELISPOT capture. For ELISPOT capture applications, a concentration range of 4.0 - 8.0 µg/mL is recommended.
Additional reported applications (for the relevant formats) include: immunoprecipitation2, immunohistochemical staining of paraformaldehyde-fixed, saponin-treated frozen tissue sections1,4-6,8, neutralization13, and immunocytochemistry.

Note: For testing human IL-2 in serum or plasma, BioLegend's LEGEND MAX™ Kit (Cat. No. 431807) is specially developed and recommended.

Clone MQ1-17H12 cross-reacts to Cat15

Application References

(PubMed link indicates BioLegend citation)
  1. Andersson J, et al. 1994. Immunology 83:16. (IHC)
  2. Abrams J, et al. 1992. Immunol. Rev. 127:5. (IP)
  3. Abrams JS. 1995. Curr. Prot. Immunol. Unit 6.20.
  4. Fernandez V, et al. 1994. Eur. J. Immunol. 24:1808. (IHC)
  5. Skansen-Saphir U, et al. 1994. Eur. J. Immunol. 24:916. (IHC)
  6. Andersson U, et al. Detection and Quantification of Gene Expression. New York:Springer-Verlag. (IHC)
  7. Prussin C, et al. 1995. J. Immunol. Methods. 188:117.
  8. Raqib R, et al. 2002. Infect. Immun. 70:3199. (IHC)
  9. Dzhagalov I, et al. 2007. J. Immunol. 178:2113. PubMed
  10. Colleton BA, et al. 2009. J Virol. 83:6288. PubMed
  11. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  12. Rout N, et al. 2010. PLoS One 5:e9787. (FC)
  13. Yeap SK, et al. 2013. BMC Complement Altern. Med. 13:145. (Neut)
  14. Wu Z, et al. 2015. J Virol. 89:6435. PubMed
  15. Maksaereekul S, et al. 2009. Vaccine. 28:3754 (FC) PubMed
Product Citations
  1. Ajith A, et al. 2021. Front Immunol. 12:687715. PubMed
  2. Pal R, et al. 2020. Curr Protoc Cytom. 92:e69. PubMed
  3. Paniskaki K, et al. 2022. Transplant Proc. 54:1455. PubMed
  4. Gartshteyn Y, et al. 2023. Life Sci Alliance. 6: . PubMed
  5. Statzu M, et al. 2023. Nat Microbiol. 8:299. PubMed
  6. Mansilla-Soto J, et al. 2022. Nat Med. 28:345. PubMed
  7. Pymm P, et al. 2022. Cell Rep. 38:110449. PubMed
  8. Yen M, et al. 2022. Cell. 185:1414. PubMed
  9. Dhariwala MO, et al. 2020. Cell Rep Med. 1:100132. PubMed
  10. Eser TM, et al. 2023. Nat Commun. 14:2952. PubMed
  11. Cella M, et al. 2019. Nat Immunol. 1.513888889. PubMed
  12. Luo X, et al. 2020. Front Immunol. 11:623. PubMed
  13. Woldemeskel BA, et al. 2020. J Clin Invest. 130:6631. PubMed
  14. Mastelic-Gavillet B, et al. 2019. J Immunother Cancer. 7:257. PubMed
  15. Costa-Martins AG, et al. 2021. Cell Rep Med. 2:100465. PubMed
  16. Lindesmith LC, et al. 2020. Cell Mol Gastroenterol Hepatol. 0.586805556. PubMed
  17. Weisberg SP, et al. 2020. Cell Reports. 29(12):3916-3932.e5.. PubMed
  18. Sung BY, et al. 2022. J Clin Invest. 132:. PubMed
  19. Kobayashi Y, et al. 2020. Int J Oncol. 999:56. PubMed
  20. van der Ploeg K, et al. 2022. Cell Rep Med. 3:100640. PubMed
  21. Balle C, et al. 2022. J Clin Invest. 132: . PubMed
  22. Fan Z, et al. 2020. EMBO Mol Med. 12:e11571. PubMed
  23. Bartolomé-Casado R, et al. 2020. Mucosal Immunol. 14:402. PubMed
  24. Nel I, et al. 2021. Diabetologia. 64(10):2306-2321. PubMed
  25. Harper JL, et al. 2020. Nat Med. 519:26. PubMed
  26. Mikkelsen S, et al. 2010. Virology. 403:163. PubMed
  27. Gao X, et al. 2022. iScience. 25:104911. PubMed
  28. Salvany‐Celades M et al. 2019. Cell Rep. 27(9):2537-2547 . PubMed
  29. Boutboul D, et al. 2018. J Clin Invest. 128:3071. PubMed
  30. Thieme CJ, et al. 2020. Cell Rep Med. 1:100092. PubMed
  31. Dykema AG, et al. 2021. J Clin Invest. 131:. PubMed
  32. Olafsdottir TA, et al. 2022. Commun Biol. 5:914. PubMed
  33. Sashihara J, et al. 2011. PLoS One. 6:e1002308. PubMed
  34. Shen C, et al. 2021. BMC Med. 19:283. PubMed
RRID
AB_315093 (BioLegend Cat. No. 500306)
AB_315093 (BioLegend Cat. No. 500307)

Antigen Details

Structure
Cytokine; 15.4 kD (Mammalian)
Bioactivity
Proliferation of T lymphocytes, B cells, anti-inflammatory, hematopoiesis, tumor surveillance
Cell Sources
T cells
Cell Targets
T cells, B cells, NK cells, LAK cells, monocytes, macrophages, oligodendrocytes
Receptors
High affinity heterotrimer of IL-2Rα/β/γ, intermediate affinity homodimer IL-2Rα (CD25; p55; Tac) and heterodimer IL-2Rβ (CD122)/γ; γ-subunit (CD132) in common with IL-4R, IL-7R, IL-13R, IL-15R
Cell Type
Tregs
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Taniguchi T, et al. 1993. Cell 73:5.
3. Nistico G. 1993. Prog. Neurobiol. 40:463.
4. Waldmann T, et al. 1993. Ann. NY Acad. Sci. 685:603.

Regulation
Upregulated by NFAT; downregulated by TCF-8 and CIF (colostrums inhibitory factor)
Gene ID
3558 View all products for this Gene ID
UniProt
View information about IL-2 on UniProt.org
Go To Top Version: 3    Revision Date: 05/23/2013

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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