- Clone
- αHUT7 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- T cell receptor Vbeta 23
- Isotype
- Mouse IgG1, κ
-
Human peripheral blood lymphocytes were stained with CD3 FITC and TCR Vβ23 (clone αHUT7) PE (top), or mouse IgG1, κ PE isotype control (bottom). -
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 349406 | 100 tests | $335.00 | |||
Vβ23 T cell receptor (TCR Vβ23) is a variant of the TCR β chain which is heterodimerized with TCR α chain and expressed on a subset of αβ+ T cells. TCR α/β recognizes peptides presented by the MHC molecules and initiates T cell mediated immune responses.
Vβ23+ T cells are stimulated by the staphylococcal enterotoxin A (SEA) and by the pyrogenic toxin superantigens (PTSAgs) SpeK, SpeL, and SpeM from streptococcus.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- T-cell leukemia line HUT78
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
- Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
-
Additional reported applications (for the relevant formats) include: stimulation of HUT78 T cells to produce IL-21.
-
Application References
(PubMed link indicates BioLegend citation) -
- Romagne F, et al. 1995. J. Immunol. Methods 186:313. (Stim)
- Rebai N, et al. 1994. P. Natl. Acad. Sci. USA 91:1529. (FC)
- Pantaleo G, et al. 1997. P. Natl. Acad. Sci. USA 94:9848. (FC)
- RRID
-
AB_10918433 (BioLegend Cat. No. 349406)
Antigen Details
- Structure
- Immunoglobulin superfamily, associate with TCR Vα chain and CD3 complex
- Distribution
-
subset of TCR αβ+ T cells
- Function
- Recognition of peptides presented by MHC molecules, responsible for T cell mediated immune response
- Ligand/Receptor
- MHC-peptide complex
- Cell Type
- T cells
- Biology Area
- Adaptive Immunity, Immunology
- Molecular Family
- TCRs
- Antigen References
-
1. Guilherme L, et al. 2007. Curr. Protein Pept. Sc. 8:39.
2. Adelman MK. 2007. Crit. Rev. Immunol. 27:221.
3. Beres SB, et al. 2002. P. Natl. Acad. Sci. USA 99:10078.
4. Smoot LM, et al. 2002. Infect. Immunol. 70:7095.
5. Lavoie PM, et al. 2005. Int. Immunol. 17:931. - Gene ID
- 6957 View all products for this Gene ID
- UniProt
- View information about TCR Vbeta23 on UniProt.org
Other Formats
View All TCR Vβ23 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| PE anti-human TCR Vβ23 | αHUT7 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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PE anti-human TCR Vβ23
Human peripheral blood lymphocytes were stained with CD3 FIT... 