PE anti-LAT Phospho (Tyr171) Antibody

Pricing & Availability
Clone
A20005D (See other available formats)
Regulatory Status
RUO
Other Names
Linker For Activation Of T Cells; P36-38
Isotype
Mouse IgG2b, κ
A20005D_-PE_Phospho_Antibody_030923
Jurkat cells untreated (negative control, open histogram), or treated with 5 mM of H2 O2 for 3 minutes (positive control, solid histogram) were fixed and permeabilized using True Phos Perm Buffer (Cat. No. 425401) and intracellularly stained with PE anti-LAT Phospho (Tyr 171) (clone A20005D), or PE Mouse IgG2b, κ isotype control (open histogram, dashed line) (representative histogram for untreated and treated cells) (Cat. No. 400314).
  • A20005D_-PE_Phospho_Antibody_030923
    Jurkat cells untreated (negative control, open histogram), or treated with 5 mM of H2 O2 for 3 minutes (positive control, solid histogram) were fixed and permeabilized using True Phos Perm Buffer (Cat. No. 425401) and intracellularly stained with PE anti-LAT Phospho (Tyr 171) (clone A20005D), or PE Mouse IgG2b, κ isotype control (open histogram, dashed line) (representative histogram for untreated and treated cells) (Cat. No. 400314).
  • A20005D_-PE_Phospho_Antibody_2_030923
    Peripherical blood mononuclear cells (PBMC) untreated (left), or treated with 5mM of H2 O2 for 3 minutes (right) were fixed and permeabilizated using True Phos Perm Buffer (Cat. No. 425401), and then intracellularly stained with PE anti-LAT Phospho (Tyr 171) (clone A20005D), and Alexa Fluor® 488 anti-human CD3 (Cat. No. 317310). Quadrants were set base on their respective isotype control.
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946605 25 tests $165.00
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946606 100 tests $435.00
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Description

LAT, or Linker for Activation of T cells, is a transmembrane protein expressed mainly in T cells and a limited number of other immune cells such as natural killer cells, mast cells, and immature B cells. As an adaptor protein, phosphorylated LAT facilitates the recruitment of other signaling proteins and functions as a nucleating site for multiprotein signaling complexes. These signaling complexes can propagate TCR, or T Cell antigen Receptors, and activate downstream effectors, which trigger T cell proliferation or cytokine expression.

LAT (Tyr171) is one of the nine tyrosines in LAT conserved between human, mice, and rat. LAT (Tyr171) is necessary for the activation of PI 3-kinase, a protein that regulates the recruitment of other proteins that facilitate in T-cell receptor signal transduction such as PLC-gamma1 and Sos1. When LAT (Tyr171) and (Tyr191) is phosphorylated by ZAP-70, Gads can bind directly to LAT (Tyr171) and (Tyr191) and mediate the recruitment of SLP-76. 

Technical data sheet

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Synthetic peptide corresponding to human LAT phosphorylated at Tyr 171
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

When this clone is used in WB at a dilution lower than 1:1000 it may show non-specific bands at 50 kD and above.

This clone has been tested by ICC on H2O2 treated and untreated Jurkat, with three fix and permeabilization methods (100% methanol, 4% PFA plus methanol and 4% PFA plus Triton X-100). All three methods can be used for ICC staining, but the PFA plus Triton X-100 fixation/permeabilization method produced the best staining.

This clone has only been tested on True-PhosTM perm buffer (Cat. No. 425401) by ICFC

RRID
AB_2936765 (BioLegend Cat. No. 946605)
AB_2936765 (BioLegend Cat. No. 946606)

Antigen Details

Structure
LAT is a 262 amino acid protein with a predicted molecular weight of 36-38 kD.
Distribution

Plasma membrane/T Cells

Function
Plasma membrane/T Cells
Cell Type
Mast cells, NK cells, T cells
Biology Area
Cell Biology, Immunology, Signal Transduction
Molecular Family
Phospho-Proteins
Antigen References
  1. Balagopalan L. et al., 2010, Cold Spring Harb Perspect Biol., 2(8): a005512
  2. Balagopalan L. et al., 2020, PLoS One, 15(2): e0229036
  3. Lin J. and Weiss A., 2001, J Biol Chem., 276(31): 29588-95
  4. Paz et. al., 2001, Biochem J., 356, 461-71
  5. Zhang W. et al., 2000, J Biol Chem., 275(30): 23355-61
Gene ID
27040 View all products for this Gene ID
UniProt
View information about LAT Phospho Tyr171 on UniProt.org
Go To Top Version: 1    Revision Date: 03/09/2023

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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