PE anti-mouse 4-1BB Ligand (CD137L) Antibody

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

BALB/c mouse B lymphoma line 2PK-3

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

The TKS-1 antibody inhibits binding of soluble 4-1BB to 4-1BBL in vitro. Additional reported applications (for the relevant formats) include: in vitro blocking the binding of 4-1BB to 4-1BBL¹. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 107106) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 107104) or biotinylated anti-rat IgG second step (Cat. No. 405402), followed by SAv-PE (Cat. No. 405204)). The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 107108).

Application References

(PubMed link indicates BioLegend citation)

1. Akiba H, et al. 2000. J. Exp. Med. 191:375. (Block)
2. Futagawa T, et al. 2002. Int. Immunol. 14:275.
3. Nishimoto H, et al. 2005. Blood 106:4241. (FC) PubMed
4. De Keersmaecker B, et al. 2011. J Leukoc Biol. 89:989. PubMed.
5. Zhao Y, et al. 2012. Immuno Lett. 141:220. PubMed.

Product Citations

1. Balakrishnan PB, et al. 2022. Nano Res. 15:2300. PubMed
2. Wu R, et al. 2022. Nat Immunol. 23:1536. PubMed
3. Li H, et al. 2022. Cancer Cell. 40:36. PubMed
4. An HW, et al. 2022. Cell Rep. 41:111878. PubMed
5. Jin SM, et al. 2023. Nat Nanotechnol. :. PubMed
6. Martin S, et al. 2016. PLoS One. 11: 0155189. PubMed
7. Clement M, et al. 2016. PLoS Pathog. 12:e1006050. PubMed
8. Hegde S, et al. 2020. Cancer Cell. 37(3):289-307. PubMed
9. Laspidea V, et al. 2022. JCI Insight. 7:. PubMed
10. Trefzer A, et al. 2021. Cell Reports. 34(6):108748. PubMed
11. Puigdelloses M, et al. 2021. J Immunother Cancer. 9:. PubMed
12. Knox T, et al. 2019. Sci Rep. 9:6136. PubMed
13. Nishimoto H, et al. 2005. Blood. 106:4241. PubMed

RRID

AB_2256408 (BioLegend Cat. No. 107105)

Antigen Details

Structure

NGF/TNF superfamily, 97 kD

Distribution

Activated B cells and T cells, macrophages, and dendritic cells

Function

T cell costimulation

Ligand/Receptor

4-1BB (CDw137)

Cell Type

B cells, Dendritic cells, Macrophages, T cells

Biology Area

Costimulatory Molecules, Immunology

Molecular Family

CD Molecules

Antigen References

1. Pollok KE, et al. 1995. Eur. J. Immunol. 25:488.
2. DeBenedette MA, et al. 1997. J. Immunol. 158:551.
3. Pollok KE, et al. 1993. J. Immunol. 150:771.
4. Pollok KE, et al. 1994. Eur. J. Immunol. 24:367.
5. Goodwin RG, et al. 1993. Eur. J. Immunol. 23:2631.

Gene ID

21942 View all products for this Gene ID

UniProt

View information about CD137L on UniProt.org

Related Protocols

• Cell Surface Flow Cytometry Staining Protocol

Other Formats