PE anti-mouse CD18 Antibody

Pricing & Availability
Clone
M18/2 (See other available formats)
Regulatory Status
RUO
Other Names
integrin β2, β2 integrin, ITGB2
Isotype
Rat IgG2a, κ
M18/2
C57BL/6 splenocytes stained with M18/2 PE
  • M18/2
    C57BL/6 splenocytes stained with M18/2 PE
Compare all formats See PE spectral data
Cat # Size Price Quantity Check Availability
101407 50 µg $86.00
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101408 200 µg $280.00
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Description

CD18 is a 95 kD protein, also known as integrin β2 subunit. It is expressed on all leukocytes. CD18, in association with integrin α chain CD11a, CD11b, and CD11c forms LFA-1, Mac-1, and αXβ2, respectively, and plays an important role in leukocytes adhesion. The CD18 integrin complexes bind ICAM-1 (CD54), ICAM-2 (CD102), ICAM-3 (CD50), iC3b, and fibrinogen. The M18/2 antibody is able to block tumor cell metastasis and enhance cell adhesion.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse CTL glycoprotein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1,2, Western blotting1, in vitro and in vivo blocking of tumor cells metastasis or stimulation of cell-adhesion2-6, and immunohistochemical staining7 of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 101417-101422).

Application References

(PubMed link indicates BioLegend citation)
  1. Sanchez-Madrid, et al. 1983. J. Exp. Med. 158:586. (IP, WB)
  2. Zahalka MA, et al. 1993. J. Immunol. 150:4466. (IP, Block, Stim)
  3. Zahalka MA, et al. 1994. Int. Immunol. 6:917. (Block, Stim)
  4. Ishida Y, et al. 1994. Cell. Immunol. 155:414. (Block, Stim)
  5. Isobe M, et al. 1992. Science 255:1125. (Block, Stim)
  6. Driessens MHE, et al. 1996. J. Leukoc. Biol. 60:758. (Block, Stim)
  7. Barlow SC, et al. 2004. Amer. J. Pathol. 165:1849. (IHC)
Product Citations
  1. Yang M, et al. 2015. J Appl Physiol. 119: 219-222. PubMed
  2. Swidergall M, et al. 2019. Cell Rep. 28:423. PubMed
  3. Kumar A, et al. 2017. J Neuroinflammation . 10.1186/s12974-017-0819-4. PubMed
  4. Omori S, et al. 2021. Cell Reports. 34(6):108734. PubMed
  5. Simonetti G, et al. 2013. J Exp Med. 210:2887. PubMed
  6. Horiguchi H, et al. 2019. Genes Dev. 33:1641. PubMed
  7. Ross EC, et al. 2021. Cell Mol Life Sci. 78:5197. PubMed
  8. Wu G, et al. 2022. Wellcome Open Res. 6:134. PubMed
  9. Wittner J, et al. 2022. Mucosal Immunol. 15:668. PubMed
  10. Yang M, et al. 2015. J Appl Physiol. 119: 77 - 82. PubMed
  11. Richardson ET, et al. 2015. PLoS One. 10: 1371. PubMed
  12. Hasenberg M, et al. 2011. PLoS One. 6:e17314. PubMed
  13. Break T, et al. 2016. Infect Immun . 84(12):3302-3312. PubMed
  14. Silva N, et al. 2016. J Immunol. 196: 2591 - 2601. PubMed
  15. Ross EC, et al. 2022. Front Immunol. 13:950914. PubMed
RRID
AB_312816 (BioLegend Cat. No. 101407)
AB_312816 (BioLegend Cat. No. 101408)

Antigen Details

Structure
Integrin family, associates with CD11a, CD11b, CD11c, 95 kD
Distribution

All leukocytes

Function
Adhesion
Ligand/Receptor
ICAM-1 (CD54), ICAM-2 (CD102), ICAM-3 (CD50), iC3b, fibrinogen
Cell Type
Leukocytes, Tregs
Biology Area
Cell Adhesion, Cell Biology, Immunology, Innate Immunity
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Anderson DC, et al. 1987. Annu. Rev. Med. 38:175.
3. Wilson RW, et al. 1993. J. Immunol. 151:1571.

Gene ID
16414 View all products for this Gene ID
UniProt
View information about CD18 on UniProt.org
Go To Top Version: 2    Revision Date: 03/07/2013

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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