PE anti-mouse CD195 (CCR5) Antibody

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Armenian Hamster

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

CCR5 is expressed at low density on activated cells. For successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 107006) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 107004) or biotinylated anti-Armenian hamster IgG (Cat. No. 405501) second step, followed by SAv-PE (Cat. No. 405204)).

Application References

(PubMed link indicates BioLegend citation)

1. Mao A, et al. 2005. J. Immunol. 175:5146. (FC) PubMed
2. Ishida Y, et al. 2007. Am J Pathol.170:843.(FC) PubMed
3. Zeiser Z, et al. 2008. Blood 111:453. (FC) PubMed
4. Sharma R, et al. 2009. J. Immunol.. 183:3212 (FC) PubMed
5. Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed

Product Citations

1. Zhuang Z, et al. 2021. J Exp Med. 218:00:00. PubMed
2. Diao N, et al. 2016. Sci Rep. 6:34672. PubMed
3. Mariani SA, et al. 2019. Immunity. 50:1439. PubMed
4. He W et al. 2018. Immunity. 49(6):1175-1190 . PubMed
5. Mencarelli A, et al. 2016. Sci Rep. 6:30802. PubMed
6. Zhang L, et al. 2021. Clin Transl Med. 11:e464. PubMed
7. Chatterjee S et al. 2017. Cell metabolism. 27(1):85-100 . PubMed
8. Nacer A, et al. 2014. PLoS Pathog. 10:1004528. PubMed
9. Oliveira AC et al. 2017. eLife. 6 pii: e30883. PubMed
10. Meunier I, et al. 2015. PLoS One. 10: 0138055. PubMed
11. Di Pilato M, et al. 2021. Cell. 184(17):4512-4530.e22. PubMed
12. Gaylo‐Moynihan A et al. 2019. Immunity. 51(2):298-309 . PubMed
13. Graham JB, et al. 2017. Curr Protoc Mouse Biol. 7:221. PubMed
14. Fend L, et al. 2014. Cancer Immunol Res. 2:1163. PubMed
15. Geng S, et al. 2016. Nat Commun. 7:13436. PubMed
16. Richardson M, et al. 2014. PLoS Negl Trop Dis. 8:2825. PubMed

RRID

AB_313300 (BioLegend Cat. No. 107005)
AB_313300 (BioLegend Cat. No. 107006)

Antigen Details

Structure

β-chemokine receptor, 45 kD

Distribution

Macrophages, T cell subset, heart, spleen, liver

Function

Lymphocyte chemotaxis and transendothelial migration during inflammation, signaling through seven transmembrane-spanning G proteins

Ligand/Receptor

RANTES, MCP-1, MIP-1α, and MIP-1β

Cell Type

Dendritic cells, Macrophages, T cells

Biology Area

Immunology, Innate Immunity

Molecular Family

CD Molecules, Cytokine/Chemokine Receptors, GPCR

Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Napolitano M, et al. 1990. J. Exp. Med. 172:285.
3. Meyer A, et al. 1996. J. Biol. Chem. 271:14445.
4. Boring, et al. 1996. J. Biol. Chem. 271:7551.

Gene ID

12774 View all products for this Gene ID

UniProt

View information about CD195 on UniProt.org

Related Protocols

• Cell Surface Flow Cytometry Staining Protocol

Other Formats