- Clone
- QA19A49 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- IL-2Rα, Ly-43, p55, and Tac
- Isotype
- Rat IgG2a, κ
-
C57BL/6 mouse splenocytes were stained with anti-mouse CD4 Alexa Flour® 488 and anti-mouse CD25 recombinant (clone QA19A49) PE (left) or rat IgG2a, κ isotype control PE (right).
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 162103 | 25 µg | $80.00 | |||
| 162104 | 100 µg | $219.00 | |||
CD25 is a 55 kD glycoprotein also known as the low affinity IL-2Rα. CD25 is expressed on activated T and B cells, thymocyte subsets, pre-B cells, and T regulatory cells. In association with CD122 (IL-2Rβ) and CD132 (common γ chain), CD25 forms the high affinity signaling IL-2 receptor.
Product Details
- Verified Reactivity
- Mouse
- Antibody Type
- Recombinant
- Host Species
- Rat
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
- Concentration
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
-
This clone binds to mouse CD25, also known as the low affinity IL-2Rα.
- RRID
-
AB_2894644 (BioLegend Cat. No. 162103)
AB_2894644 (BioLegend Cat. No. 162104)
Antigen Details
- Structure
- CD25 is a 55 kD glycoprotein.
- Distribution
-
B cells, T cells, Thymocytes, Tregs
- Function
- IL-2 receptor
- Ligand/Receptor
- IL-2
- Cell Type
- B cells, T cells, Tregs
- Biology Area
- Immunology
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors
- Antigen References
-
1. Taniguchi T, et al. 1993. Cell 73:5.
2. Waldmann TA. 1991. J. Biol. Chem. 266:2681.
3. Read S, et al. 2000. J. Exp. Med. 192:295.
4. Lowenthal JW, et al. 1985. J. Immunol. 135:3988. - Gene ID
- 16184 View all products for this Gene ID
- UniProt
- View information about CD25 on UniProt.org
Other Formats
View All CD25 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-mouse CD25 Recombinant Antibody | QA19A49 | FC |
| PE anti-mouse CD25 Recombinant Antibody | QA19A49 | FC |
| APC anti-mouse CD25 Recombinant Antibody | QA19A49 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-mouse CD25 Recombinant Antibody
C57BL/6 mouse splenocytes were stained with anti-mouse CD4 F... -
PE anti-mouse CD25 Recombinant Antibody
C57BL/6 mouse splenocytes were stained with anti-mouse CD4 A... -
APC anti-mouse CD25 Recombinant Antibody
C57BL/6 mouse splenocytes were stained with anti-mouse CD4 A...