- Clone
- A10 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- CD314
- Isotype
- Armenian Hamster IgG
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C57BL/6 mouse splenocytes stained with A10 PE and DX5 APC
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 115605 | 50 µg | $122.00 | |||
| 115606 | 200 µg | $353.00 | |||
NKG2D is a lectin-like type II transmembrane protein also known as CD314. It is expressed on NK cells, a subset of CD8+ T cells, γ/δ T cells and NK1.1+ T cells, as well as in vitro induced LAK cells. NKG2D serves as a stimulatory immunoreceptor to activate NK cells via the non-covalently associated DAP10 or DAP12 adaptor. Several molecules have been identified as the ligands for NKG2D, including minor histocompatibility molecule, H60, UL16-binding protein-like transcript 1 (Mult1, and a family of retinoic acid early transcript 1 (Rae1) in mice, MHC class-I chain-related protein A (MICA), MICB, and UL16-binding proteins (ULBPs) in humans. present in both mice and humans. NKG2D ligands trigger cytokine (IFN-γ, GM-CSF, TNF-α, MIP1β and others) and granzyme release from NK cells. The A10 antibody has been reported to costimulate NK cells.
Product Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Armenian Hamster
- Immunogen
- Soluble mouse NKG2D
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
-
Additional reported applications include: blocking and inducing redirected cell lysis1,2. To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 (Cat. No. 101301/101302), is recommended prior to immunofluorescent staining. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 115606) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-Armenian hamster IgG second step (Cat. No. 405501), followed by SAv-PE (Cat. No. 405204)). The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 115610).
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Application References
(PubMed link indicates BioLegend citation) -
- Gilfillan S, et al. 2002. Nat Immunol. 3:1150.
- Ho EL, et al. 2002. J. Immunol. 169:3667.
- Product Citations
-
- RRID
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AB_313659 (BioLegend Cat. No. 115605)
AB_313659 (BioLegend Cat. No. 115606)
Antigen Details
- Structure
- C-type lectin
- Distribution
-
NK, NK T
- Function
- NK cytolytic killing of target cells expressing NKG2D ligands, costimulation NK cells
- Receptors
- Minor histocompatibility molecule H60, retinoic acid early inducible 1 (RAE-1) family
- Cell Type
- NK cells, NKT cells
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Vance RE, et al. 1999. J. Exp. Med. 190:1801.
2. Vance RE, et al. 1998. J. Exp. Med. 188:1841.
3. Lohwasser S, et al. 1999. Eur. J. Immunol. 29:755.
4. Yokoyama WM. 1998. Curr. Opin. Immunol. 10:298.
5. Gilfillan S, et al. 2002. Nat. Immunol. 3:1150.
6. Ho EL, et al. 2002. J. Immunol. 169:3667. - Gene ID
- 27007 View all products for this Gene ID
- UniProt
- View information about CD314 on UniProt.org
Other Formats
View All CD314 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| PE anti-mouse CD314 (NKG2D) | A10 | FC |
Compare Data Across All Formats
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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PE anti-mouse CD314 (NKG2D)
C57BL/6 mouse splenocytes stained with A10 PE and DX5 APC