- Clone
- RH1 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- BGR, βGR, CD369
- Isotype
- Rat IgG1, κ
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Thioglycolate-induced C57BL/6 mouse peritoneal cells were stained with anti-mouse CD369 (Dectin-1/CLEC7A, clone RH1) PE (filled histogram) or rat IgG1, κ PE isotype control (open histogram). Data shown was gated on the F4/80-positive macrophage population.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 144303 | 25 µg | $117.00 | |||
| 144304 | 100 µg | $335.00 | |||
CD369 (Dectin-1), also known as CLEC7A, is a 43 kD type II transmembrane protein. The extracellular portion of Dectin-1 contains one C-type lectin domain, and its cytoplasmic tail has one immunoreceptor tyrosine-based activation motif (ITAM). Dectin-1 binds β-1,3-linked and β-1,6-glucans (abundant in the cell wall of fungi and yeast), mediates phagocytosis, and induces cytokine production and oxidative burst. It is also involved in TLR2-mediated NF-kB activation. Dectin-1 is expressed by macrophages, neutrophils, dendritic, and Langerhans cells.
Product Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Product Citations
-
- RRID
-
AB_2561500 (BioLegend Cat. No. 144303)
AB_2561500 (BioLegend Cat. No. 144304)
Antigen Details
- Structure
- 43 kD, type II transmembrane protein, 1 C-type lectin domain, 1 ITAM motif
- Distribution
- Macrophages, dendritic cells, Langerhans cells
- Function
- Phagocytosis, induces cytokine production and oxidative burst
- Ligand/Receptor
- β-1,3-linked and β-1,6-linked glucans
- Cell Type
- Macrophages, Dendritic cells, Langerhans cells, Neutrophils
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- CD Molecules
- Antigen References
-
1. Iliev ID, et al. 2012. Science 336:1314.
2. Saijo S and Iwakura Y. 2011. Int. Immunol. 23:467.
3. Reid DM, et al. 2009. Curr. Opin. Immunol. 21:30.
4. Saijo S, et al. 2007. Nat. Immunol. 8:39. - Gene ID
- 56644 View all products for this Gene ID
- UniProt
- View information about CD369 on UniProt.org
Other Formats
View All CD369 (Dectin-1, CLEC7A) Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| PE anti-mouse CD369 (Dectin-1/CLEC7A) | RH1 | FC |
| Purified anti-mouse CD369 (Dectin-1/CLEC7A) | RH1 | FC |
| APC anti-mouse CD369 (Dectin-1/CLEC7A) | RH1 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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PE anti-mouse CD369 (Dectin-1/CLEC7A)
Thioglycolate-induced C57BL/6 mouse peritoneal cells were st... -
Purified anti-mouse CD369 (Dectin-1/CLEC7A)
Mouse Dectin-1-transfected cells were stained with purified ... -
APC anti-mouse CD369 (Dectin-1/CLEC7A)
Thioglycolate-induced C57BL/6 mouse peritoneal cells were st...