PE anti-mouse CD3ε Antibody

Pricing & Availability
Clone
145-2C11 (See other available formats)
Regulatory Status
RUO
Other Names
CD3ε, T3, CD3
Isotype
Armenian Hamster IgG
1_145-2c11
C57BL/6 mouse splenocytes were stained with CD3e (clone 145-2C11) PE and CD19 FITC.
  • 1_145-2c11
    C57BL/6 mouse splenocytes were stained with CD3e (clone 145-2C11) PE and CD19 FITC.
  • 2_145-2C11_PE_CD3e_Antibody_2_040122
    C57BL/6 mouse splenocytes were stained with CD3e (clone 145-2C11) PE (filled histogram) or Armenian hamster IgG PE isotype control (open histogram).
Compare all formats See PE spectral data
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100307 50 µg $44.00
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100308 200 µg $114.00
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Description

CD3ε is a 20 kD transmembrane protein, also known as CD3 or T3. It is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3ε forms a TCR complex by associating with the CD3δ, γ and ζ chains, as well as the TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
H-2Kb-specific mouse cytotoxic T lymphocyte clone BM10-37
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone 145-2C11 is useful for in vitro blocking of target-specific CTL-mediated cell lysis1, as well as T cell activation assays, inducing proliferation and cytokine production1,2,7,12,16. It also induces apoptosis in immature thymocytes32,  and in vivo T cell depletion8-10. Additional reported applications (for relevant formats of this clone) include: immunoprecipitation1, immunohistochemical staining14,15 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, Western blotting4, complement-mediated cytotoxicity6, in vitro and in vivo stimulation of T cells1,2,7,12,16, immunofluorescent staining5, and in vivo T cell depletion8-10. The 145-2C11 antibody has been reported to block the binding of 17A2 antibody to CD3 epsilon-specific T cells11. Clone 145-2C11 is not recommended for formalin-fixed paraffin embedded sections. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 100314). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 100340) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Leo O, et al. 1987. P. Natl. Acad. Sci. USA 84:1374. (IP, Activ, Block)
  2. Kruisbeek AM, et al. 1991. In Current Protocols in Immunology. 3.12.1. (Activ)
  3. Duke RC, et al. 1995. Current Protocols in Immunology. 3.17.1.
  4. Salvadori S, et al. 1994. J. Immunol. 153:5176. (WB)
  5. Payer E, et al. 1991. J. Immunol. 146:2536. (IF)
  6. Jacobs H, et al. 1994. Eur. J. Immunol. 24:934. (CMCD)
  7. Vossen ACTM, et al. 1995. Eur. J. Immunol. 25:1492. (Activ)
  8. Henrickson M, et al. 1995. Transplantation 60:828. (Deplete)
  9. Kinnaert P, et al. 1996. Transpl. Int. 9:386. (Deplete)
  10. Han WR, et al. 1999. Transpl. Immunol. 7:207. (Deplete)
  11. Miescher GC, et al. 1989. Immunol. Lett. 23:113. (Block)
  12. Terrazas LI, et al. 2005. Intl. J. Parasitology. 35:1349. (Activ)
  13. Ko SY, et al. 2005. J. Immunol. 175:3309.
  14. Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC-F)
  15. Tilley SL, et al. 2007. J. Immunol. 178:3208. (IHC-F)
  16. Wang W, et al. 2007. J. Immunol. 178:4885. (Activ)
  17. Xiao S, et al. 2007. J. Exp. Med. 204:1691.
  18. Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245. (FC) PubMed.
  19. Curtsinger JM, et al.2005. J. Immunol. 175:4392. PubMed
  20. Guo Y, et al. 2008. Blood 112:480. PubMed
  21. Kenna TJ, et al. 2008. Blood 111:2091.
  22. Perchonock CE, et al. 2007. J. Immunol. 179:1768. PubMed
  23. Perchonock GE, et al. 2006. Mol. Cell. Biol. 26:6005. PubMed
  24. Kanaya T, et al. 2008. Am. J. Physiol. Gastrointest. Liver Physiol. 295:G273. PubMed
  25. de Koning BA, et al. 2006. Int. Immunol. 18:941. PubMed
  26. Schulteis RD, et al. 2008. Blood 295:G273. PubMed
  27. Qi Q, et al. 2009. Blood 114:564. PubMed
  28. Helmersson S, et al. 2013. Am J Pathol. 9440:123. Pubmed
  29. Wu S, et al. 2014. Clin Vaccine Immunol. 21:156. PubMed
  30. Yan J, et al. 2014. Vaccine. 32:2833. PubMed
  31. Guiterrez DA, et al. 2014. Diaebetes. 63:3827. PubMed
  32. Shi YF, et al. 1991. J Immunol. 146:3340. (Apop)
Product Citations
  1. Grigsby SM, et al. 2021. Cancers (Basel). 13:. PubMed
  2. Wang L, et al. 2022. ACS Pharmacol Transl Sci. 5:110. PubMed
  3. Iberg CA, et al. 2022. Cell Rep. 39:110657. PubMed
  4. Ocaña-Guzman R, et al. 2022. Cells. 11: . PubMed
  5. Wang R, et al. 2023. NPJ Vaccines. 8:6. PubMed
  6. Zhao K, et al. 2023. Front Immunol. 14:1101769. PubMed
  7. Hajjar S, et al. 2022. Cell Death Differ. 29:585. PubMed
  8. Ramdas B, et al. 2022. Mol Ther. 30:2505. PubMed
  9. Zong P, et al. 2022. Nat Cardiovasc Res. 1:344. PubMed
  10. Elazar A, et al. 2022. Elife. 11:. PubMed
  11. Guo H, Cooper S, Friedman A, et al. 2017. PLoS One. 10.1371/journal.pone.0150809. PubMed
  12. Chang YS, et al. 2020. Int J Mol Sci. 21:00. PubMed
  13. Logan K Smith et al. 2018. Immunity. 48(2):299-312 . PubMed
  14. Wu J et al. 2017. Immunity. 47(6):1114-1128 . PubMed
  15. Jayachandran R, et al. 2019. Immunity. 50:152. PubMed
  16. Sierro F, et al. 2017. Immunity. 47:374. PubMed
  17. Yu X, et al. 2020. Nat Commun. 11:1110. PubMed
  18. Olofsen PA, et al. 2022. Cells. 11:. PubMed
  19. Wu Y, et al. 2023. Nat Cancer. 4:382. PubMed
  20. Sambandam A, et al. 2023. Heliyon. 9:e14238. PubMed
  21. Touré H, et al. 2023. PLoS Pathog. 19:e1011257. PubMed
  22. He Y, et al. 2023. Biomolecules. 13:. PubMed
  23. Gao Y, et al. 2023. iScience. 26:106729. PubMed
  24. Taddeo A, et al. 2022. NPJ Vaccines. 7:82. PubMed
  25. Thelin MA, et al. 2017. Diabetes. 66:2220. PubMed
  26. Piepke M, et al. 2021. J Neuroinflammation. 18:265. PubMed
  27. Qin Y, et al. 2021. Cell Rep. 37:109968. PubMed
  28. MacDonald K, et al. 2014. J Immunol. 192:3180. PubMed
  29. Grødeland G, et al. 2020. Front Immunol. 11:431. PubMed
  30. Leier A, et al. 2022. Mol Ther Nucleic Acids. 28:261. PubMed
  31. Acharya N, et al. 2020. Immunity. 53(3):658-671.e6. PubMed
  32. Hiraishi Y, et al. 2018. Sci Rep. 8:18052. PubMed
  33. Wang G, et al. 2021. Nat Commun. 12:5733. PubMed
  34. Motozono C, et al. 2014. Microbes Infect. 16:320. PubMed
  35. D Kim, S Lee, H Jun 2016. Immunol Cell Biol. 10.1038/icb.2016.98. PubMed
  36. Okuniewska M, et al. 2021. Cell Reports. 36(2):109368. PubMed
  37. Bartleson JM, et al. 2020. Nat Immunol. 1384:21. PubMed
  38. Dave K et al. 2017. eLife. 6 pii: e23382. PubMed
  39. LaFleur MW, et al. 2019. Nat Immunol. 20:1335. PubMed
  40. Xu X, et al. 2021. J Nanobiotechnology. 19:376. PubMed
  41. Vazquez S, et al. 2015. Exp Hematol. 43: 578-585. PubMed
  42. Sarapulov AV, et al. 2020. Front Immunol. 11:599. PubMed
  43. Pan W et al. 2017. Immunity. 47(2):284-297 . PubMed
  44. Zhang B, et al. 2022. J Exp Med. 219:. PubMed
  45. Yoshida K, et al. 2022. Sci Rep. 12:17276. PubMed
  46. Vitoriano-Souza J, et al. 2012. PLoS One. 7:e40745. PubMed
  47. Zhang B, et al. 2016. Mol Cell. 63: 976-89. PubMed
  48. Davidson S, et al. 2020. Cell Reports. 31(7):107628. PubMed
  49. Kotaki R, et al. 2020. Sci Rep. 10:13554. PubMed
  50. Flommersfeld S, et al. 2021. Immunity. :. PubMed
  51. Qiu Z, et al. 2016. Sci Rep. 6:31936. PubMed
  52. Gaudreault N, et al. 2013. Am J Pathol. 2:605. PubMed
  53. He L, et al. 2019. iScience. 0.790972222. PubMed
  54. Ma J, et al. 2016. Proc Natl Acad Sci U S A. 113: 450 - 458. PubMed
  55. Ruer-Laventie J, et al. 2020. Bio Protoc. e3531:10. PubMed
  56. Wen Y, et al. 2020. Hypertension. 869:75. PubMed
  57. Ringel AE, et al. 2020. Cell. 183(7):1848-1866.e26. PubMed
  58. Siamishi I, et al. 2020. Cell Reports. 31(11):107756. PubMed
  59. Leal AS, et al. 2019. Sci Rep. 5.286111111. PubMed
  60. Qi S et al. 2016. eLife. 5 pii: e14756. PubMed
  61. Malik A et al. 2018. Immunity. 49(3):515-530 . PubMed
  62. Bourque J, et al. 2021. Heliyon. 7:e08311. PubMed
  63. Boulay A, et al. 2015. J Neurosci. 35:4427. PubMed
  64. Faust HJ, et al. 2020. J Clin Invest. 130:5493. PubMed
  65. Abe M, et al. 2020. Clin Exp Immunol. 303:199. PubMed
  66. LaFleur MW, et al. 2019. Nat Commun. 10:1668. PubMed
  67. Park HB, et al. 2020. Nat Microbiol. 1319:5. PubMed
  68. Kim P, et al. 2015. Infect Immun . 83: 2992 - 3002. PubMed
  69. Chulpanova DS, et al. 2021. Biology (Basel). 10:. PubMed
  70. Chen RJ, et al. 2022. iScience. 25:105595. PubMed
  71. Guo Y, et al. 2013. Infect Immun . 81:3923. PubMed
  72. Kretschmer L, et al. 2020. Nat Commun. 0.536805556. PubMed
  73. Ha TC, et al. 2020. Hum Gene Ther. 32:458. PubMed
  74. Abdelfattah N, et al. 2022. Nat Commun. 13:767. PubMed
  75. Shibuya M, et al. 2021. iScience. 24:103131. PubMed
  76. Ben-Yehuda H, et al. 2021. Mol Neurodegener. 16:39. PubMed
  77. Wang L, et al. 2021. Sci Adv. 7:eabj4796. PubMed
  78. Unsinger J, et al. 2021. J Leukoc Biol. 109:697. PubMed
  79. Martin R, et al. 2014. PLoS One. 9:110609. PubMed
  80. Cecchinato V, et al. 2017. J Immunol. 198(1):184-195. PubMed
  81. Gomzikova MO, et al. 2020. Pharmaceutics. 12:00. PubMed
  82. Kristensen LK, et al. 2020. Mol Imaging Biol. 1.625694444. PubMed
  83. Mah-Som AY, et al. 2021. Cell Reports. 35(9):109209. PubMed
  84. Bergquist M, et al. 2014. J Infect. 69:113. PubMed
  85. Mohrin M, et al. 2021. Aging Cell. 20:e13313. PubMed
  86. Kuo P, et al. 2016. J Am Heart Assoc. 5: 002610. PubMed
  87. Ji XJ, et al. 2019. Mediators Inflamm. 2019:2750528. PubMed
  88. Celis‐Gutierrez J et al. 2019. Cell Rep. 27(11):3315-3330 . PubMed
  89. Tang-Huau TL, et al. 2021. Viruses. 13: . PubMed
  90. Hara T, et al. 2021. Cancer Cell. 39(6):779-792.e11. PubMed
  91. Hu KH, et al. 2020. Nat Methods. 1.286805556. PubMed
  92. Simoni L, et al. 2020. Cell Rep. 33:108330. PubMed
  93. Celià-Terrassa T, et al. 2017. Nat Cell Biol. 19:711. PubMed
  94. Dourcy M, et al. 2020. Mucosal Immunol. 13:799. PubMed
  95. Bansal K, et al. 2017. Nat Immunol. 18:263-273. PubMed
  96. Evavold CL, et al. 2021. Cell. 184(17):4495-4511.e19. PubMed
  97. Dyer DP et al. 2019. Immunity. 50(2):378-389 . PubMed
  98. Sethuraman SN, et al. 2020. Theranostics. 10:3397. PubMed
  99. Artinger M, et al. 2021. Int J Mol Sci. 22: . PubMed
  100. Avgustinova A, et al. 2021. Cell Stem Cell. . PubMed
  101. Campisi L, et al. 2016. Nat Immunol. 10.1038/ni.3512. PubMed
  102. Qi S, et al. 2020. Theranostics. 10:1814. PubMed
  103. Kim I et al. 2015. Brain and behavior. 5(12):e00403 . PubMed
  104. Kubli SP, et al. 2019. Nat Commun. 10:2678. PubMed
RRID
AB_312672 (BioLegend Cat. No. 100307)
AB_312672 (BioLegend Cat. No. 100308)

Antigen Details

Structure
Ig superfamily, forms CD3/TCR complex with CD3δ, γ and ζ subunits and TCR (α/β and γ/δ), 20 kD
Distribution

Thymocytes (differentiation dependent), mature T cells, NK-T cells

Function
TCR signal transduction, T cell activation, antigen recognition
Ligand/Receptor
Peptide antigen/MHC-complex
Cell Type
NKT cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules, TCRs
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Davis MM. 1990. Annu. Rev. Biochem. 59:475.
3. Weiss A, et al. 1994. Cell 76:263.

Gene ID
12501 View all products for this Gene ID
UniProt
View information about CD3epsilon on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

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