PE anti-mouse IL-9 Antibody

Pricing & Availability
Clone
RM9A4 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-9, P40, Mast cell growth factor (MCGF), Megakaryoblast growth factor, T cell growth factor-3 (TCGF-3)
Isotype
Rat IgG1, κ
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    FACS sorted naive CD4 T cells (CD44lo, CD25-) were cultured on plates coated with 1 µg/ml anti-CD3 and 10 µg/ml anti-CD28 in the presence of 10 ng/ml IL-4 and 5 ng/ml TGFβ for 3 days. Intracellular staining was performed following a 4h restimulation with PdBU/ionomycin/brefeldin. Data kindly provided by: Dr. Brigitta Stockinger, PhD
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514103 25 µg $183.00
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514104 100 µg $347.00
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Description

IL-9 is a potent, T cell-derived, T cell growth factor which can also enhance mast cell activity and IL-3- or IL-4- dependent proliferation of bone marrow-derived mast cells. IL-9 synergizes with erythropoietin to promote erythroid colony formation. IL-9 induces high affinity IgE receptor expression and granzyme A and B in murine T helper clones.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant full length mouse IL-9
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The IL-9 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

See the protocol for Th9 polarization of mouse splenocytes.

Application References

(PubMed link indicates BioLegend citation)
  1. Angkasekwinai P, et. al. 2010. Nat. Immunol. 11:250. (ICFC) PubMed
  2. Chang HC, et al. 2010. Nat. Immunol. 11:527. (ICFC) PubMed
  3. Takami M, et al. 2012. J Immunol. 188:4369. PubMed
  4. Reynolds LA, et al. 2012. J. Immunol. 189:1113. PubMed
  5. Kuwahara M, et al. 2014. Nat Commun. 5:3555. PubMed
Product Citations
  1. Katrin Schaper‐Gerhardt et al. 2018. British journal of pharmacology. 1111/bph. PubMed
  2. Schwartz DM et al. 2019. Immunity. 50(1):106-120 . PubMed
  3. Kuwahara M, et al. 2014. Nat Commun. 5:3555. PubMed
  4. Zhou Y, et al. 2011. J Immunol. 186:4415. PubMed
  5. Jiang Y, et al. 2019. J Immunother Cancer. 7:28. PubMed
  6. Mikami N, et al. 2013. J Immunol. 190:4046. PubMed
  7. Gomez-Rodriguez J, et al. 2016. Nat Commun. 7:10857. PubMed
  8. Alhosaini K, et al. 2021. Brain Sci. :11. PubMed
  9. Kim B, et al. 2010. Proc Natl Acad Sci U S A. 107:8742. PubMed
  10. Prins J, et al. 2015. Biol Reprod. 93: 95. PubMed
  11. Tan C, et al. 2010. J Immunol. 185:6795. PubMed
  12. Zhao Y, et al. 2016. Nat Commun. 7:12368. PubMed
  13. Roy S, et al. 2021. Nat Commun. 12:3182. PubMed
  14. Chen J, et al. 2021. Oncol Lett. 21:409. PubMed
  15. Lu Y, et al. 2018. Cancer Cell. 33:1048. PubMed
RRID
AB_2126639 (BioLegend Cat. No. 514103)
AB_2126639 (BioLegend Cat. No. 514104)

Antigen Details

Structure
Cytokine; 30-40 kD (Mammalian)
Bioactivity
Potentiates production of IgG, IgM, and IgE by IL-4-induced B lymphocytes; regulates granzyme family proteases; enhances proliferation of bone marrow mast cells with IL-3; production of IL-6 by mast cells
Cell Sources
IL-2 activated TH2 lymphocytes
Cell Targets
T lymphocytes, erythroid precursors
Receptors
IL-9R
Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Quesniaux V, et al. 1992. Research Immunology 143:385.
3. Renauld J, et al. 1993. Adv. Immunol. 54:79.
4. Yang Y, et al. 1992. Leuk. Lymphoma 8:441.

Regulation
Upregulated by IL-1 after TCR activation, TNF-α; downregulated by antibodies to IL-2
Gene ID
16198 View all products for this Gene ID
UniProt
View information about IL-9 on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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