- Clone
- TR310 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- T cell receptor Vβ7 chain
- Isotype
- Rat IgG2b, κ
-
BALB/c splenocytes stained with CD3 (145-2C11) APC and TR310 PE
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 118307 | 25 µg | $104.00 | |||
| 118308 | 100 µg | $214.00 | |||
The TR310 antibody reacts with the mouse T cell receptor Vβ7 segment . T cells expressing the α/β T cell receptor use clonally distributed TCRs to recognize cell-bound antigens such as peptide/MHC complexes and viral superantigens. The α/β TCR is an oligomeric TCR complex composed of covalently bound α and β chains involved in antigen recognition and the non-covalently associated monomorphic proteins CD3δ, γ, ε, and ζ chains. The TR310 antibody reacts with one such clonally distributed TCR β chain known as Vβ7. Vβ7 is expressed on a small subpopulation of both CD4+ and CD8+ T cells. The TR310 antibody has been used for the identification of Vβ7 expressing T cells by flow cytometry and immunoprecipitation.
Product Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- T cell lymphoma TK-1
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Additional reported applications (for the relevant formats) include immunoprecipitation2.
-
Application References
(PubMed link indicates BioLegend citation) -
- Ito K, et al. 2000. Proc. Natl. Sci. USA 97:740.
- Okada CY, et al. 1990. J. Immunol. 144:3473.
- Product Citations
-
- RRID
-
AB_893631 (BioLegend Cat. No. 118307)
AB_893631 (BioLegend Cat. No. 118308)
Antigen Details
- Structure
- Ig superfamily member, TCR α/β with CD3 forms the CD3/TCR complex
- Distribution
-
Expressed on a subpopulation of CD4+ and CD8+ T cells bearing the Vβ7 T cell receptor segment
- Function
- Antigen recognition, T cell activation
- Ligand/Receptor
- Peptide bound to MHC
- Receptors
- Peptide bound to MHC
- Cell Type
- T cells
- Biology Area
- Adaptive Immunity, Immunology
- Molecular Family
- TCRs
- Antigen References
-
1. Okada CY, et al. 1990. J. Immunol. 144:3473.
- Gene ID
- 21577 View all products for this Gene ID
- UniProt
- View information about TCR Vbeta7 on UniProt.org
Other Formats
View All TCR Vβ7 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| FITC anti-mouse TCR Vβ7 | TR310 | FC |
| PE anti-mouse TCR Vβ7 | TR310 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
FITC anti-mouse TCR Vβ7
Balb/c mouse splenocytes stained with CD3 (145-2C11) APC and... -
PE anti-mouse TCR Vβ7
BALB/c splenocytes stained with CD3 (145-2C11) APC and TR310...