- Clone
- A21012B (See other available formats)
- Regulatory Status
- RUO
- Other Names
- NF-kB p65, RelA,P65, NFKB3, Nuclear Factor NF-Kappa-B P65 subunit, Transcription Factor P65, NF-Kappa-B Transcription Factor P65, RELA Proto-Oncogene NF-KB subunit
- Isotype
- Mouse IgG1, κ
-
Peripheral blood mononuclear cells untreated (negative control, open histogram), or treated with Cell Activation Cocktail™ (without Brefeldin A) (Cat. No. 423301) (positive control, filled histogram) were fixed and permeabilized using True-Phos™ Perm Buffer (Cat. No. 425401), and then intracellularly stained with PE anti-NF-κB p65 Phospho (Ser529) (clone A21012B) or PE mouse IgG1, κ Isotype Control (open histogram, dashed line) (representative histogram for untreated or treated cells) (Cat. No. 400111).
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 614153 | 25 tests | $150.00 | |||
| 614154 | 100 tests | $380.00 | |||
NF-κB p65, also known as NFKB3 and RelA, is one of five members of the nuclear factor κB (NF-κB)/Rel family. Inactive NF-κB p65 is sequestered in the cytoplasm as a complex with IκB inhibitory proteins. Upon activation of the NF-κB pathway, IκB proteins are degraded, thus releasing NF-κB p65 to the nucleus. Binding of NF-κB p65 to transcriptional cofactors is induced via phosphorylation on serines 276, 529, 536, and 471. As a transcription factor, NF-κB p65 is vital to cell proliferation, immune response, survival, and apoptosis. Through regulation of chondrocyte and osteoblast differentiation and survival, NFκB p65 has a crucial role in skeletal development. Overexpressed in several cancers, NF-κB p65 functions as an oncogene and has been implicated in tumorigenesis, metastasis, tumor angiogenesis, and chemoresistance.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Synthetic peptide corresponding to human NF-κB p65 phosphorylated at serine 529
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Phos™ Perm Buffer in Cell Suspensions Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
-
When testing by ICC, we recommend fixation with 4% PFA followed by permeabilization with Triton X-100.
- RRID
-
AB_3097548 (BioLegend Cat. No. 614153)
AB_3097548 (BioLegend Cat. No. 614154)
Antigen Details
- Structure
- NF-κB p65 is a 551 amino acid protein with a predicted molecular weight of 60 kD.
- Distribution
-
Ubiquitously expressed/ Cytoplasm and Nucleus
- Function
- Transcription, RAS pathway, host-virus interaction, oncogene
- Interaction
- NF-kappa-B, TLE5, TLE1, TP53BP2, GSK3B, MAP3K14, HDAC3, AKIP1, FOXP3
- Biology Area
- Cell Biology, Immuno-Oncology, Immunology, Innate Immunity, Transcription Factors
- Molecular Family
- Innate Immune Signaling, Phospho-Proteins, Protein Kinases/Phosphatase
- Antigen References
-
- Giridharan S and Srinivasan M. 2018. J Inflamm Res. 11:407-419.
- Ren C, et al. 2022. Cell Death Differ. 29:381-392.
- Yu S, et al. 2020. FEBS Lett. 594:13681378.
- Gene ID
- 5970 View all products for this Gene ID
- UniProt
- View information about NF-kappaB p65 on UniProt.org
Other Formats
View All NF-κB p65 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-NF-κB p65 Phospho (Ser529) | A21012B | WB,ICC,ICFC |
| PE anti-NF-κB p65 Phospho (Ser529) | A21012B | ICFC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-NF-κB p65 Phospho (Ser529)
HeLa cells were fixed with 4% PFA Fixation Buffer (Cat. No. ... 
Whole cell extracts (15 µg total protein) from untreated HeL... 
HeLa cells treated with 20 ng/ml TNFα and 100 nM Calyculin A... -
PE anti-NF-κB p65 Phospho (Ser529)
Peripheral blood mononuclear cells untreated (negative contr...