- Clone
- 4D10.2 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Spleen tyrosine kinase, protein tyrosine kinase SYK
- Isotype
- Mouse IgG2a, κ
-
Human peripheral blood lymphocytes were surface stained with CD3 Brilliant Violet 421™ then treated with the True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) and intracellularly stained with anti-Syk (clone 4D10.2) PE (left) or mouse IgG2a, κ PE isotype control (right) -
Human peripheral blood lymphocytes were surface stained with CD56 APC then treated with the True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) and intracellularly stained with anti-Syk (clone 4D10.2) PE (left) or mouse IgG2a, κ PE isotype control (right). -
Human peripheral blood lymphocytes were surface stained with CD19 APC then treated with the Nuclear Factor Fixation and Permeabilization Buffer Set and intracellularly stained with anti-Syk (clone 4D10.2) PE (left) or mouse IgG2a, κ PE isotype control (right).
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 644303 | 25 tests | $141.00 | |||
| 644304 | 100 tests | $347.00 | |||
Syk is a cytoplasmic 72 kD non-receptor tyrosine kinase that is widely expressed in hematopoietic cells. The Syk kinase associates with a number of proteins including c-Src, Lck, Lyn, Fyn, Vav1, STAT3, SHP1, Grb2, and LAT to couple immunoreceptors to downstream signaling events mediating cellular proliferation, differentiation, and phagocytosis. Syk is modified by phosphorylation on multiple tyrosine sites
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human Syk peptide aa 314-339
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
- Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
-
This product is for in vitro research use only. It is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for commercial purposes, contact The Regents of the University of California.
Intracellular stained with Nuclear Factor Fixation and Permeabilization Buffer Set for Flow Cytometry. -
Application References
(PubMed link indicates BioLegend citation) -
- Van Oers, et al. 1995. J. Exp. Med. 182:1585
- Chu DH, et al. 1999. J. Immunol. 163:2610.
- RRID
-
AB_2565303 (BioLegend Cat. No. 644303)
AB_2565303 (BioLegend Cat. No. 644304)
Antigen Details
- Structure
- Non-receptor associated tyrosine kinase, contains 2 SH2 domains, 72 kD
- Distribution
-
Cytoplasmic protein, widely expressed in hematopoietic cells
- Function
- Couples immunoreceptor to downstream signaling events that mediate cellular responses including proliferation, differentiation, and phagocytosis
- Biology Area
- Cell Biology, Immunology, Signal Transduction
- Antigen References
-
1. Taniguchi T, et al. 1991. J. Biol. Chem. 266:15790.
2. Toyabe SL, et al. 2001.Immunology 103:164. - Gene ID
- 6850 View all products for this Gene ID
- UniProt
- View information about Syk on UniProt.org
Other Formats
View All Syk Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-Syk | 4D10.2 | WB,IP,ICFC,IHC-P |
| PE anti-Syk | 4D10.2 | ICFC |
| APC anti-Syk | 4D10.2 | ICFC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Purified anti-Syk
Total cell lysates (15 µg total protein) from A549 (negative... 
Jurkat cell extract was resolved by electrophoresis, transfe... 
IHC staining using purified anti-Syk (clone 4D10.2) on forma... 
IHC staining using purified anti-Syk (clone 4D10.2) on form... -
PE anti-Syk
Human peripheral blood lymphocytes were surface stained with... 
Human peripheral blood lymphocytes were surface stained with... 
Human peripheral blood lymphocytes were surface stained with... -
APC anti-Syk
Human peripheral blood lymphocytes were surface stained with... 