- Clone
- 5F5 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Spleen tyrosine kinase, protein tyrosine kinase SYK
- Isotype
- Mouse IgG1, κ
-
C57BL/6 mouse splenocytes were surface stained with CD19 Brilliant Violet 421™, then treated with the Nuclear Factor Fixation and Permeabilization Buffer Set, and intracellularly stained with anti-Syk (clone 5F5) PE (top) or mouse IgG1, κ PE isotype control (bottom). -
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 646003 | 25 µg | $153.00 | |||
| 646004 | 100 µg | $360.00 | |||
Syk is a cytoplasmic 72 kD non-receptor tyrosine kinase that is widely expressed in hematopoietic cells. The Syk kinase associates with a number of proteins including c-Src, Lck, Lyn, Fyn, Vav1, STAT3, SHP1, Grb2, and LAT to couple immunoreceptors to downstream signaling events mediating cellular proliferation, differentiation, and phagocytosis. Syk is modified by phosphorylation on multiple tyrosine sites.
Product Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Mouse Syk peptide aa306-333
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.03 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
-
This product is for in vitro research use only. It is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for commercial purposes contact The Regents of the University of California.
-
Application References
(PubMed link indicates BioLegend citation) -
- Chu D, et al. 1999. J. Immunol. 163:2610.
- Ackermann JA, et al. 2015. J Immunol. 194:4650. PubMed
- Product Citations
-
- RRID
-
AB_2565305 (BioLegend Cat. No. 646003)
AB_2565305 (BioLegend Cat. No. 646004)
Antigen Details
- Structure
- Non-receptor associated tyrosine kinase, contains 2 SH2 domains, 72 kD
- Distribution
-
Cytoplasmic protein, widely expressed in hematopoietic cells
- Function
- Couples immunoreceptor to downstream signaling events that mediate cellular responses including proliferation, differentiation, and phagocytosis
- Biology Area
- Cell Biology, Signal Transduction
- Antigen References
-
1. Taniguchi T, et al. 1991. J. Biol. Chem. 266:15790.
2. Toyabe SL, et al. 2001.Immunology 103:164. - Gene ID
- 6850 View all products for this Gene ID
- UniProt
- View information about Syk on UniProt.org
Other Formats
View All Syk Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-Syk | 5F5 | WB,IP |
| PE anti-Syk | 5F5 | ICFC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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