- Clone
- TRIM-4 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- T cell receptor-interacting molecule
- Isotype
- Mouse IgG2a, κ
-
Human peripheral blood lymphocytes were treated with True-Nuclear™ Transcription Factor Buffer Set. Cells were then stained with TRIM (clone TRIM-4) PE (filled histogram) or mouse IgG2a, κ PE isotype control (open histogram).
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 627203 | 25 tests | $159.00 | |||
| 627204 | 100 tests | $347.00 | |||
TRIM is a 30 kD disulfide-linked protein also known as T cell receptor interacting molecule. TRIM is a type III membrane protein containing a very short extracellular domain (8 amino acids). TRIM is highly expressed in T cells with some expression also observed in NK cells. TRIM is a signaling adaptor molecule that links extracellular and intracellular signaling. The TRIM protein is phosphorylated by Src kinases and associates with the PI3K regulatory subunit α after phosphorylation. TRIM has also been shown to associate with TCRα, β, and ζ. The TRIM-4 monoclonal antibody recognizes human TRIM and has been shown to be useful for Western blotting, intracellular staining by flow cytometry, and immunofluorescence staining.
Product Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Partial recombinant human TRIM (amino acids 29-186)
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
- Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
-
Additional reported applications (for the relevant formats) include: intracellular flow cytometry1, ELISA1, and immunofluorescence microscopy2.
Clone TRIM-4 has been shown to cross-react to mouse TRIM1. -
Application References
(PubMed link indicates BioLegend citation) -
- Huynh T, et al. 2001. Scand. J. Immunol. 54:146.
- Kirchgessner H, et al. 2001. J. Exp. Med. 193:1269.
- RRID
-
AB_2565761 (BioLegend Cat. No. 627203)
AB_2565761 (BioLegend Cat. No. 627204)
Antigen Details
- Structure
- Type III membrane protein, 30 kD disulfide-linked dimeric phosphoprotein
- Distribution
- T cells, lower expression levels in NK cells
- Function
- Links extracellular and intracellular signaling pathways in T cell activation
- Interaction
- PI3K regulatory subunit α, TCRα, β, and ζ.
- Modification
- Tyrosine phosphorylation by Src kinases
- Cell Type
- T cells, NK cells
- Biology Area
- Cell Biology, Immunology, Signal Transduction
- Antigen References
-
1. Bruyns E, et al. 1998. J. Exp. Med. 188:561.
2. Hubener C, et al. 2000. Immunogenetics 51:154. - Gene ID
- 50852 View all products for this Gene ID
- UniProt
- View information about TRIM on UniProt.org
Other Formats
View All TRIM Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-TRIM | TRIM-4 | WB,ICC,ICFC |
| PE anti-TRIM | TRIM-4 | ICFC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-TRIM
Total cell lysates (15 µg total protein) from Molt-4 and U87... -
PE anti-TRIM
Human peripheral blood lymphocytes were treated with True-Nu...