PE/Cyanine5 anti-human CD18 Antibody

Pricing & Availability
Clone
TS1/18 (See other available formats)
Regulatory Status
RUO
Workshop
V AS162
Other Names
Integrin β2 subunit, LFA -1 β subunit, β2 integrin, ITGB2
Isotype
Mouse IgG1, κ
TS1slash18_PECy5_061807
Human peripheral blood lymphocytes stained with TS1/18 PE/Cyanine5
  • TS1slash18_PECy5_061807
    Human peripheral blood lymphocytes stained with TS1/18 PE/Cyanine5
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Cat # Size Price Quantity Check Availability
302109 25 tests $129.00
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Description

CD18 is a 90-95 kD type I transmembrane protein also known as integrin β2 subunit, LFA-1 β subunit, and β2 integrin. CD18 non-covalently associates with CD11a, CD11b or CD11c. CD18 is expressed on all leukocytes. CD18 and associated α chains function in adhesion and signaling in hematopoietic cells.

Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
Pig
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: inhibition of cell adhesion and migration3,4. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) (Cat. No. 302116) is recommended for functional assays.

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Kishimoto T, et al. 1997. Leucocyte Typing VI. Garland Press. London.
  3. Van Epps DE, et al. 1989. J. Immunol. 143:3207. (Block)
  4. Meerschaert J, et al. 1994. J. Immunol. 152:1915. (Block)
  5. Sithu SD, et al. 2007. J. Biol. Chem. doi:10.1074/jbc.M611273200.
  6. Sommaggio R, et al. 2012. J. Immunol. 188:2075. PubMed
  7. Valenzuela NM, et al. 2013. J. Immunol. 190:6635. PubMed
Product Citations
  1. McKillop W, et al. 2009. J Leukoc Biol. 86:851. PubMed
  2. Schmidt T, et al. 2012. J Leukoc Biol. 91:791. PubMed
  3. Mastrogiovanni M, et al. 2022. Sci Adv. 8:eabl5942. PubMed
  4. Hegewisch-Solloa E, et al. 2021. J Immunol. 207:950. PubMed
  5. Navarro-Hernandez IC, et al. 2020. FEBS J. 287:3449. PubMed
RRID
AB_314227 (BioLegend Cat. No. 302109)

Antigen Details

Structure
Integrin, type I transmembrane glycoprotein, associates with CD11a, CD11b, or CD11c, 90-95 kD
Distribution

Leukocytes

Function
Adhesion and signaling in hematopoietic system
Ligand/Receptor
ICAMs
Cell Type
Leukocytes, Tregs
Biology Area
Cell Adhesion, Cell Biology, Immunology, Innate Immunity
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Anderson D, et al. 1987. Annu. Rev. Med. 38:175.
2. Springer T. 1994. Cell 76:301.

Gene ID
3689 View all products for this Gene ID
UniProt
View information about CD18 on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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