PE/Cyanine5 Streptavidin

Product Details

Verified Reactivity

Human, Mouse, Rat, All Species

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

Streptavidin is conjugated with PE/Cyanine5 under optimal conditions.

Concentration

0.2 mg/ml (concentration relates to the Streptavidin only component of the conjugate)

Storage & Handling

The Streptavidin-PE/Cyanine5 solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

ICFC - Verified

Recommended Usage

Each lot of this Streptavidin-PE/Cyanine5 is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular molar mass of streptavidin independent of any additional molecular mass that might be added by the PE/Cyanine5 conjugation. For immunofluorescent staining, the recommended use of this reagent is = 0.125 µg per 10⁶ cells in 100 µl staining volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

Streptavidin-Phycoerythrin/Cyanine5 (PE/Cyanine5) is useful as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. The average molecular weight of Streptavidin-PE/Cyanine5 is 480 kD and Streptavidin alone is 52 kD.

Application References

(PubMed link indicates BioLegend citation)

1. Wong KA, et al. 2008. J. Immunol. 15:180. PubMed
2. Qi Q, et al. 2009. Blood PubMed
3. Imada M, et al. 2009. Int immunol. PubMed
4. Sawa Y, et al. 2009. Immunity. 30:447. PubMed
5. Song R, et al. 2010. J. Virol. PubMed
6. Abdel-Latif A, et al. 2010. Exp Hematol. 38:1131. PubMed
7. De Monte L, et al. 2011. J. Exp Med. 208:469. PubMed
8. Xu B, et al. 2012. PLoS One. 7:e44719. PubMed
9. Su ML, et al. 2014. PLoS One. 9:98823. PubMed
10. Chen A, et al. 2014. J Immunol. 193:35. PubMed
11. Soni C, et al. 2014. J Immunol. 193:4400. PubMed
12. Wong EB, et al. 2015. J Immunol. 194:4130. PubMed

Product Citations

1. Ogishi M, et al. 2023. J Exp Med. 220: . PubMed
2. Muliaditan T, et al. 2021. Cell Rep Med. 2:100457. PubMed
3. Song R, et al. 2010. J Virol. 84:6935. PubMed
4. Ogishi M, et al. 2022. J Exp Med. 219:. PubMed
5. Abdel-Latif A, et al. 2010. Exp Hematol. 38:1131. PubMed
6. Rodriguez K 2008. J Immunol. 180:716. PubMed
7. Pereira JL, et al. 2021. Transl Oncol. 14:101125. PubMed
8. Domeier P, et al. 2016. J Exp Med. 213: 715 - 732. PubMed
9. JI B, et al. 2016. Cell Death Differ. 23:759-75. PubMed
10. Soni C, et al. 2014. J Immunol. 193:4400. PubMed
11. Su M, et al. 2014. PLoS One. 9:98823. PubMed
12. Imada M, et al. 2009. Int Immunol. 1.674305556. PubMed
13. Suliman HB, et al. 2022. iScience. 25:103535. PubMed
14. Cuenca M, et al. 2016. J Immunol. 196: 726 - 737. PubMed
15. Xu B, et al. 2012. PLoS One. 7:e44719. PubMed
16. Chen A, et al. 2014. J Immunol. 193:35. PubMed
17. Qi Q, et al. 2009. Blood. 114:564. PubMed
18. Wong E, et al. 2015. J Immunol. 194:4130. PubMed
19. Pennington LF, et al. 2021. Nat Commun. 12:7069. PubMed
20. Chiem K, et al. 2021. Microbiol Spectr. 9:e0160121. PubMed
21. Sawa Y, et al. 2009. Immunity. 30:447. PubMed
22. Monte L, et al. 2011. J Exp Med. 208:469. PubMed
23. Li ZG, et al. 2020. Chin Med J (Engl). 1943:133. PubMed
24. Hayashi K, et al. 2020. Nat Commun. 4.832638889. PubMed

Antigen Details

Gene ID

NA

UniProt

View information about Biotin on UniProt.org

Related Protocols

• Cell Surface Flow Cytometry Staining Protocol