PE/Cyanine7 anti-human CD34 Antibody

Pricing & Availability
Clone
561 (See other available formats)
Regulatory Status
RUO
Other Names
Gp105-120, My10, Mucosialin
Isotype
Mouse IgG2a, κ
561_PECyanine7_CD34_Antibody_1_102523
Human peripheral blood mononuclear cells were stained with CD45 APC and CD34 (clone 561) PE/Cyanine7 (left) or PE/Cyanine7 mouse IgG2a, κ isotype control (right). Data shown was gated on CD14- cell population.
  • 561_PECyanine7_CD34_Antibody_1_102523
    Human peripheral blood mononuclear cells were stained with CD45 APC and CD34 (clone 561) PE/Cyanine7 (left) or PE/Cyanine7 mouse IgG2a, κ isotype control (right). Data shown was gated on CD14- cell population.
Compare all formats See PE/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability
343615 25 tests $159.00
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343616 100 tests $358.00
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Description

CD34, also known as gp105-120, is a type I monomeric sialomucin-like glyco-phosphoprotein with an approximate molecular weight of 105-120 kD. It is selectively expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nervous tissue. CD34 is a commonly used marker for identifying human hematopoietic stem/progenitor cells. Based on different sensitivities, four groups of epitopes of CD34 have been described. CD34 mediates cell adhesion and lymphocytes homing through binding to L-selectin and E-selectin ligands.

Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The 561 antibody recognizes a class III group epitope, which is resistant to sialidase/glycolyprotease and chymopapain treatment.

Application References

(PubMed link indicates BioLegend citation)
  1. Croockewit AJ, et al. 1998. Scand. J. Immunol. 47:82.
  2. Rosenzweig M, et al. 2001. J. Med. Primatol. 30:36.
Product Citations
  1. Wilkes MC, et al. 2022. Exp Hematol. 111:66. PubMed
  2. Downes DJ, et al. 2021. Nat Genet. 53:1606. PubMed
  3. Xu Y, et al. 2021. Stem Cell Res Ther. 12:112. PubMed
  4. Forsberg MH, et al. 2021. Stem Cell Res Ther. 12:459. PubMed
  5. Isobe T, et al. 2022. Nat Commun. 13:4501. PubMed
  6. Chou DB, et al. 2022. Nat Commun. 13:6021. PubMed
RRID
AB_2629725 (BioLegend Cat. No. 343615)
AB_2629725 (BioLegend Cat. No. 343616)

Antigen Details

Structure
105-120 kD single chain mucin-like glycoprotein
Distribution

Hematopoietic stem/progenitor cells, bone marrow stromal cells, endothelial cells, embryonic fibroblasts

Function
Cell adhesion
Ligand/Receptor
L-selecin, E-selectin
Cell Type
Endothelial cells, Fibroblasts, Hematopoietic stem and progenitors
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience, Stem Cells
Molecular Family
CD Molecules
Gene ID
947 View all products for this Gene ID
UniProt
View information about CD34 on UniProt.org
Go To Top Version: 2    Revision Date: 11/01/2016

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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