PE/Cyanine7 anti-human CD43 Antibody

Product Details

Verified Reactivity

Human

Reported Reactivity

African Green, Baboon, Cynomolgus

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

MV4-11 cell line

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application References

(PubMed link indicates BioLegend citation)

1. Rosenkranz AR, et al. 1993. Immunology. 80:431
2. Skubitz KM, et al. 1998. J. Leukoc. Biol. 64:800

Product Citations

1. Iqneibi S, et al. 2023. Front Immunol. 1239148:14. PubMed
2. Coley WD et al. 2018. F1000Research. 2018 Mar 14 [revised 2018 Jan 1] 0.5125. PubMed
3. Matsuo S, et al. 2021. PLoS One. e0247595:16. PubMed

RRID

AB_2563698 (BioLegend Cat. No. 343207)
AB_2563698 (BioLegend Cat. No. 343208)

Antigen Details

Structure

Single chain, type I transmembrane glycoprotein with abundant O-glycosylation and sialylation sites, 95-130 kD.

Distribution

Most hematopoietic cells, including peripheral T cells, NK cells, granulocytes, monocytes, thymocytes, platelets, plasma cells, activated B cells, and hemotopoietic progenitor cells.

Function

Plays dual roles in cell-cell adhesion and anti-adhesion, costimulation of cell activation and survival, inducing apoptosis of T cells and hematopoietic progenitors.

Ligand/Receptor

CD54, Siglec-1

Biology Area

Immunology

Molecular Family

Adhesion Molecules, CD Molecules

Antigen References

1. Neil Barclay A, et al. 1997. The Leucocyte Antigen Facts Book:Second Edition. Page 238
2. Killeen A, et al. 1987. EMBO J. 6:4029.
3. Thurman EC, et al. 1998. Intl. Immunol. 10:691.
4. Onami TM, et al. 2002. J. Immunol. 168:6022.
5. Tong J, et al. 2004. J. Exp. Med. 199:1277.
6. Jones AT, et al. 1994. J. Immunol. 153:3426.

Gene ID

6693 View all products for this Gene ID

UniProt

View information about CD43 on UniProt.org

Related Protocols

• Cell Surface Flow Cytometry Staining Protocol

Other Formats