PE/Cyanine7 anti-human CD80 Antibody

Pricing & Availability
Clone
2D10 (See other available formats)
Regulatory Status
RUO
Workshop
VI CD80.1
Other Names
B7-1, B7, BB1
Isotype
Mouse IgG1, κ
2D10_PECy7_100809
Human B-cell Burkitt's lymphoma cell line Raji stained with 2D10 PE/Cyanine7
  • 2D10_PECy7_100809
    Human B-cell Burkitt's lymphoma cell line Raji stained with 2D10 PE/Cyanine7
Compare all formats See PE/Cyanine7 spectral data
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305217 25 tests $141.00
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305218 100 tests $305.00
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Description

CD80, also known as B7-1, B7, and BB1, is a 60 kD single chain type I glycoprotein belonging to the immunoglobulin superfamily. CD80 is expressed on activated B and T cells, macrophages, and dendritic cells. CD80 binds to CD28 and CD152 (CTLA-4). Along with CD86, CD80 plays a critical role in regulation of T cell activation. The interaction of CD80 with CD28 provides a potent costimulatory signal for T cell activation through the CD3 complex, while its interaction with CTLA-4 provides an inhibitory signal for T cell activation.

Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: in vitro blocking of T cell activation, immunohistochemical staining of acetone-fixed frozen tissue sections2, immunoprecipitation, and Western blotting3. The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 305245 & 305246).

Application References

(PubMed link indicates BioLegend citation)
  1. Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
  2. Battifora M. 1998. J. Clin. Endocr. Metab. 83:4130. (IHC)
  3. Van der Merwe PA, et al. 1997. J. Exp. Med. 185:3. (WB)
  4. Jayakumar A, et al. 2008. Infect. Immun. 76:2138. PubMed
  5. Schubert DA, et al. 2012. J. Exp Med. 209:335. PubMed
  6. Wen T, et al. 2014. J Immunol. 192:5481. PubMed
Product Citations
  1. Avital G, et al. 2022. Cell Rep. 41:111477. PubMed
  2. Korniotis S, et al. 2022. J Cell Sci. 135:. PubMed
  3. García-García A, et al. 2023. Bioact Mater. 24:174. PubMed
  4. Zhou R, et al. 2020. Immunity. S1074-7613(20)30333-2.. PubMed
  5. Magalhães L, et al. 2015. PLoS Negl Trop Dis. 10: 0003816. PubMed
  6. Zhong J, et al. 2018. Sci Adv. 4:eaas9864. PubMed
  7. Meng Y,et al. 2017. Cell Death Dis. . 10.1038/cddis.2017.505. PubMed
  8. Abe S, et al. 2020. JCI Insight. 5:00. PubMed
  9. Kotsiou E, et al. 2016. Blood. 128: 72 - 81. PubMed
  10. Neff CP et al. 2018. EBioMedicine. 30:192-202 . PubMed
  11. Cassetta L et al. 2019. Cancer Cell. 35(4):588-602 . PubMed
  12. Sarkar MK, et al. 2018. Ann Rheum Dis. 77:1653. PubMed
  13. Sureshchandra S, et al. 2021. iScience. 24:102690. PubMed
  14. Boyd A, et al. 2013. Infect Immun . 81:1420. PubMed
  15. Sabree SA, et al. 2021. J Immunother Cancer. 9: . PubMed
  16. Richardson JR, et al. 2018. Front Immunol. 2.182638889. PubMed
  17. Wei Z, et al. 2021. Nat Commun. 0.805555556. PubMed
  18. Cecotto L, et al. 2022. iScience. 25:105211. PubMed
  19. Koh WH, et al. 2020. STAR Protoc. 1:100203. PubMed
  20. Murphy DM, et al. 2022. J Clin Invest. Online ahead of print. PubMed
RRID
AB_1877254 (BioLegend Cat. No. 305217)
AB_1877254 (BioLegend Cat. No. 305218)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, 60 kD
Distribution

Activated B cells and T cells, macrophages, and dendritic cells

Function
T cell costimulation
Ligand/Receptor
CD28, CD152 (CTLA-4)
Cell Type
B cells, Dendritic cells, Macrophages, T cells, Tregs
Biology Area
Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Freeman G, et al. 1991. J. Exp. Med. 174:625.
2. Linsley P, et al. 1996. Immunity 4:535.
3. Linsley P, et al. 1991. J. Exp. Med. 174:561.

Gene ID
941 View all products for this Gene ID
UniProt
View information about CD80 on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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