- Clone
- MH9A4 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Interleukin-9, P40, Mast cell growth factor (MCGF), Megakaryoblast growth factor, T cell growth factor-3 (TCGF-3)
- Isotype
- Mouse IgG2b, κ
-
PMA+ionomycin-stimulated (6 hours, in the presence of monensin) Th2-polarized lymphocytes were stained with CD3 APC, permeabilized, and intracellularly stained with IL-9 (clone MH9A4) PE/Cyanine7 (left), or mouse IgG2b, κ PE/Cyanine7 isotype control (right).
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 507611 | 25 tests | $147.00 | |||
| 507612 | 100 tests | $323.00 | |||
IL-9 is a potent, T cell-derived, T cell growth factor which can also enhance mast cell activity and IL-3- or IL-4- dependent proliferation of bone marrow-derived mast cells. IL-9 synergizes with erythropoietin to promote erythroid colony formation. IL-9 has also been reported to protect human T cells from apoptosis induced by IL-2 withdrawal. IL-9 is upregulated in human eosinophils by TNF-α and IL1-β. IL-9 has been reported to downregulate the oxidative burst in activated human alveolar macropahges and induce TGF-β production. The MH9A4 antibody reacts with human IL-9. The MH9A4 antibody can neutralize the bioactivity of natural or recombinant IL-9.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Baculovirus-expressed, recombinant human IL-9
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
- Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
-
ELISA Capture2: The purified MH9A4 antibody is useful as the capture antibody in a human IL-9 sandwich ELISA assay, when used in conjunction with the biotinylated MH9D1 antibody as the detecting antibody.
For human IL-9 neutralization assay, the LEAF™ purified MH9D1 antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for neutralization of human IL-9 bioactivity. -
Application References
(PubMed link indicates BioLegend citation) - RRID
-
AB_2564552 (BioLegend Cat. No. 507611)
AB_2564552 (BioLegend Cat. No. 507612)
Antigen Details
- Structure
- Cytokine; 32-39 kD (Mammalian)
- Bioactivity
- Potentiates production of IgG, IgM, and IgE by IL-4-induced B lymphocytes; regulates granzyme family proteases; enhances proliferation of bone marrow mast cells with IL-3; production of IL-6 by mast cells
- Cell Sources
- IL-2 activated TH2 lymphocytes, Hodgkin's lymphoma
- Cell Targets
- T lymphocytes, erythroid precursors
- Receptors
- IL-9R
- Biology Area
- Immunology
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Quesniaux V. 1992. Research Immunology 143:385.
3. Renauld J, et al. 1993. Adv. Immunol. 54:79.
4. Yang Y. 1992. Leuk. Lymphoma 8:441. - Regulation
- Upregulated by IL-1 after TCR activation, TNF-α; downregulated by antibodies to IL-2
- Gene ID
- 3578 View all products for this Gene ID
- UniProt
- View information about IL-9 on UniProt.org
Other Formats
View All IL-9 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-human IL-9 | MH9A4 | ELISA Capture |
| PE anti-human IL-9 | MH9A4 | ICFC |
| Alexa Fluor® 647 anti-human IL-9 | MH9A4 | ICFC |
| PerCP/Cyanine5.5 anti-human IL-9 | MH9A4 | ICFC |
| PE/Cyanine7 anti-human IL-9 | MH9A4 | ICFC |
| APC anti-human IL-9 | MH9A4 | ICFC |
| TotalSeq™-B1151 anti-human IL-9 | MH9A4 | ICPG |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Purified anti-human IL-9
-
PE anti-human IL-9
Enriched human CD4+ T cells were stimulated with PMA+ionomyc... -
Alexa Fluor® 647 anti-human IL-9
PMA and ionomycin-stimulated (6 hours, in the presence of mo... -
PerCP/Cyanine5.5 anti-human IL-9
PMA and ionomycin-stimulated (6 hours, in the presence of mo... -
PE/Cyanine7 anti-human IL-9
PMA+ionomycin-stimulated (6 hours, in the presence of monens... -
APC anti-human IL-9
PMA and ionomycin-stimulated (6 hours, in the presence of mo... -
TotalSeq™-B1151 anti-human IL-9