- Clone
- QA20A06 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- SHPS-1, BI, PTPNS1, CD172 antigen-like family member A
- Isotype
- Mouse IgG1, κ
-
C57BL/6 mouse bone marrow cells were stained with anti-mouse CD11b APC and anti-mouse CD172a recombinant (clone QA20A06) PE/Cyanine7 (left) or mouse IgG1, κ PE/Cyanine7 isotype control (right). Data shown was gated on the myeloid cell population.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 108603 | 25 µg | $155.00 | |||
| 108604 | 100 µg | $365.00 | |||
CD172a, also known as SIRPα, is a type I transmembrane protein with one V-set Ig-like and two C-set Ig-like domains in the extracellular portion, and two ITIM motifs and a proline-rich region in the cytoplasmic tail. CD172a is expressed by monocytes, macrophages, myeloid cells, and neuronal tissue. The phosphorylation of SIRPα ITIMs induces the recruitment and activation of the tyrosine phosphatases PTPN6 and PTPN11, resulting in the negative regulation of several biological processes. The ligands of CD172a are CD47, SP-A, and SP-D.
Product Details
- Verified Reactivity
- Mouse
- Antibody Type
- Recombinant
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
- Concentration
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
-
Clone QA20A06 can completely block the binding of clone P84 on target cells.
- RRID
-
AB_3097458 (BioLegend Cat. No. 108603)
AB_3097458 (BioLegend Cat. No. 108604)
Antigen Details
- Structure
- Type I transmembrane protein
- Distribution
-
Monocytes, macrophages, myeloid cells, neuronal tissue
- Function
- Negative regulation of several biological processes
- Interaction
- PTPN6, PTPN11
- Ligand/Receptor
- CD47, SP-A, SP-D
- Cell Type
- Dendritic cells, Macrophages, Monocytes, Neutrophils
- Biology Area
- Cell Adhesion, Cell Biology, Immunology, Signal Transduction
- Molecular Family
- Adhesion Molecules, CD Molecules, Protein Kinases/Phosphatase
- Antigen References
-
- Zhao XW, et al. 2011. Proc Natl Acad Sci U S A. 108:18342-7.
- Verjan GN, et al. 2011. J Immunol. 187:2268-77.
- Sato-Hashimoto M, et al. 2011. J Immunol. 187:291-7.
- Raymond M, et al. 2010. Eur J Immunol. 40:3510-8.
- Gene ID
- 19261 View all products for this Gene ID
- UniProt
- View information about CD172alpha on UniProt.org
Other Formats
View All CD172a Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-mouse CD172a (SIRPα) Recombinant Antibody | QA20A06 | FC |
| PE/Cyanine7 anti-mouse CD172a (SIRPα) Recombinant Antibody | QA20A06 | FC |
| APC anti-mouse CD172a (SIRPα) Recombinant Antibody | QA20A06 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-mouse CD172a (SIRPα) Recombinant Antibody
C57BL/6 mouse bone marrow cells were stained with anti-mouse... -
PE/Cyanine7 anti-mouse CD172a (SIRPα) Recombinant Antibody
C57BL/6 mouse bone marrow cells were stained with anti-mouse... -
APC anti-mouse CD172a (SIRPα) Recombinant Antibody
C57BL/6 mouse bone marrow cells were stained with anti-mouse...