PE/Cyanine7 Streptavidin

Pricing & Availability
Regulatory Status
RUO
Other Names
SAv-PE/Cyanine7
PECyanine7_Streptavidin_Antibody_042123
Human peripheral blood lymphocytes were stained with PE/Cyanine7 streptavidin alone (open histogram) or Biotin anti-human CD3 (clone UCHT1) followed by (PE/Cyanine7) Streptavidin (filled histogram).
  • PECyanine7_Streptavidin_Antibody_042123
    Human peripheral blood lymphocytes were stained with PE/Cyanine7 streptavidin alone (open histogram) or Biotin anti-human CD3 (clone UCHT1) followed by (PE/Cyanine7) Streptavidin (filled histogram).
See PE/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability
405206 100 µg $206.00
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Description

Streptavidin binds to biotin with high affinity. Streptavidin - Phycoerythrin/Cyanine7 (PE/Cyanine7) is useful for detecting biotinylated antibodies. The excitation of PE/Cyanine7 by 488 nm laser light induces a light emission maximum of 775 nm.

Technical data sheet

Product Details

Verified Reactivity
Human, Mouse, Rat, All Species
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
Streptavidin is conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml (concentration relates to the Streptavidin only component of the conjugate)
Storage & Handling
The Streptavidin-PE/Cyanine7 solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

ICFC - Verified
Recommended Usage

Each lot of this Streptavidin-PE/Cyanine7 is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular molar mass of streptavidin independent of any additional molecular mass that might be added by the PE/Cyanine7 conjugation.  For immunofluorescent staining, the recommended use of this reagent is =0.125 µg per 106 cells in 100 µl staining volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Streptavidin - Phycoerythrin/Cyanine7 (PE/Cyanine7) is useful as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. The average molecular weight of Streptavidin-PE/Cyanine7 is 360 kD and Streptavidin alone is 52 kD.

Application References

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