PE/Dazzle™ 594 anti-human β2-microglobulin Antibody

Pricing & Availability
Clone
A17082A (See other available formats)
Regulatory Status
RUO
Other Names
β2M, β2-M, beta2-microglobulin b2-M, b2M
Isotype
Mouse IgG1, κ
A17082A_PEDazzle594_beta2_microglobulin_Antibody_101123
Human peripheral blood lymphocytes were stained with anti-human β2-microglobulin (clone A17082A) PE/Dazzle™ 594 (filled histogram) or mouse IgG1, к PE/Dazzle™ 594 isotype control (open histogram).
  • A17082A_PEDazzle594_beta2_microglobulin_Antibody_101123
    Human peripheral blood lymphocytes were stained with anti-human β2-microglobulin (clone A17082A) PE/Dazzle™ 594 (filled histogram) or mouse IgG1, к PE/Dazzle™ 594 isotype control (open histogram).
Compare all formats See PE/Dazzle™ 594 spectral data
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395723 25 tests $170.00
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395724 100 tests $375.00
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Description

β2-microglobulin (β2M) is a 12 kD nonpolymorphic Ig like protein. It is a non-membrane-anchored glycoprotein and is noncovalently associated with 39-44 kD polymorphic heavy chains of MHC class I molecules to form HLA class I antigen complex. In association with HLA class I, β2M is expressed on all leukocytes, platelets, endothelial cells, and epithelial cells. β2M plays an essential role both in governing MHC class I molecules stability and in promoting antigen binding and presenting the antigen to CD3/TCR complex of CD8+ T cells.

Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human Beta2-microglobulin
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone A17082A partially cross-blocks anti-human ß2-microglobulin clone 2M2 and does not block anti-human HLA-A,B,C clone W6/32 staining, based on in-house testing.

RRID
AB_3083393 (BioLegend Cat. No. 395723)
AB_3083393 (BioLegend Cat. No. 395724)

Antigen Details

Structure
12 KD, Ig like structure
Distribution

Leukocytes, platelets, endothelial cells, epithelial cells and other nucleated cells.

Function
Form MHC class I
Interaction
MHC class I heavy chain and other MHC I like molecules.
Cell Type
Antigen-presenting cells, Endothelial cells, Epithelial cells, Leukocytes, Platelets
Biology Area
Cancer Biomarkers, Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References
  1. Engelhard VH. 1994. Curr. Opin. Immunol. 6:13.
  2. Williams DB, et al. 1989. J. Immunol. 142:2796.
  3. Danliczyk UG and TL. Delovitch. 1994. J. Immunol. 153:3533.
  4. Williams A, et al. 2002. Tissue Antigens. 59:3.
Gene ID
567 View all products for this Gene ID
UniProt
View information about beta2-microglobulin on UniProt.org
Go To Top Version: 1    Revision Date: 10/11/2023

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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