PE/Dazzle™ 594 anti-human CD122 (IL-2Rβ) Antibody

Pricing & Availability
Clone
TU27 (See other available formats)
Regulatory Status
RUO
Workshop
V C050
Other Names
IL-2 Receptor β chain, IL-2Rβ
Isotype
Mouse IgG1, κ
TU27_PEDazzle594_CD122_Antibody_1_110317
Human peripheral blood lymphocytes were stained with CD56 APC and CD122 (clone TU27) PE/Dazzle™ 594(top) or mouse IgG1, κ PE/Dazzle™ 594 isotype control (bottom).
  • TU27_PEDazzle594_CD122_Antibody_1_110317
    Human peripheral blood lymphocytes were stained with CD56 APC and CD122 (clone TU27) PE/Dazzle™ 594(top) or mouse IgG1, κ PE/Dazzle™ 594 isotype control (bottom).
  • TU27_PEDazzle594_CD122_Antibody_2_110317
Compare all formats See PE/Dazzle™ 594 spectral data
Cat # Size Price Quantity Check Availability
339017 25 tests $159.00
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339018 100 tests $393.00
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Description

CD122 is a 70-75 kD type I transmembrane glycoprotein and member of the Ig superfamily. It is IL-2 receptor β chain also known as IL-2Rβ, which is also shared by the IL-15 receptor. CD122 is constitutively expressed by NK cells and at lower levels by a subset of T cells. Its expression is upregulated upon activation. The IL-2Rβ chain can combine with either the common γ subunit (γc, CD132) alone or with the γc subunit and the IL-2Rα subunit (CD25) to generate intermediate or high affinity IL-2 receptor complexes, respectively. CD122 expression levels can be upregulated by activation.

Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
TL-Mor cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications include (for the relevant formats) include: immunoprecipitation, blocking of IL-2 binding to CD122, and partial inhibition of IL-2 induced cell proliferation.

Application References

(PubMed link indicates BioLegend citation)
  1. Takeshita T, et al. 1989. J. Exp. Med. 169:1323.
RRID
AB_2721348 (BioLegend Cat. No. 339017)
AB_2721348 (BioLegend Cat. No. 339018)

Antigen Details

Structure
Ig superfamily, forms high affinity IL-2 receptor with CD25 and CD132 chains or intermediate affinity receptor with CD132 alone, 70-75 kD
Distribution

NK cells, T subset

Function
Critical component of IL-2 and IL-15 signaling
Ligand/Receptor
IL-2, IL-15
Cell Type
NK cells, T cells, Tregs
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers Wiley-Liss A John Wiley & Sons Inc, Publication
2. Minami Y, et al. 1993. Annu. Rev. Immunol. 11:245.
3. Suzuki H, et al. 1995. Science 268:1472.

Gene ID
3560 View all products for this Gene ID
UniProt
View information about CD122 on UniProt.org
Go To Top Version: 0    Revision Date: 11/03/2017

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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