PE/Dazzle™ 594 anti-human IL-10 Antibody

Pricing & Availability
Clone
JES3-19F1 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin 10, B cell derived T cell growth factor (B-TCGF), Cytokine synthesis inhibitory factor (CSIF), T-cell growth inhibitory factor (TGIF)
Isotype
Rat IgG2a, κ
JES3-19F1_PEDZL594_IL-10_Antibody_1_111116
LPS-stimulated (in the presence of monensin, 18 hours) human peripheral blood mononuclear cells were surface stained with CD14 APC, then fixed with Fixation Buffer permeabilized with Permeabilization Wash Buffer and intracellularly stained with anti-human IL-10 (clone JES3-19F1) PE/Dazzle™ 594 (top) or rat IgG2a, κ PE/Dazzle™ 594 isotype control (bottom). Data shown was gated on monocyte population.
  • JES3-19F1_PEDZL594_IL-10_Antibody_1_111116
    LPS-stimulated (in the presence of monensin, 18 hours) human peripheral blood mononuclear cells were surface stained with CD14 APC, then fixed with Fixation Buffer permeabilized with Permeabilization Wash Buffer and intracellularly stained with anti-human IL-10 (clone JES3-19F1) PE/Dazzle™ 594 (top) or rat IgG2a, κ PE/Dazzle™ 594 isotype control (bottom). Data shown was gated on monocyte population.
  • JES3-19F1_PEDZL594_IL-10_Antibody_2_111116
Compare all formats See PE/Dazzle™ 594 spectral data
Cat # Size Price Quantity Check Availability
506811 25 tests $141.00
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506812 100 tests $352.00
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Description

IL-10 was originally described as Cytokine Synthesis Inhibitory Factor (CSIF) by virtue of its ability to inhibit cytokine production by Th1 clones. IL-10 shares over 80% sequence homology with the Epstein-Barr virus protein BCRFI. The biological activities of IL-10 include inhibition of macrophage-mediated cytokine synthesis, suppression of the delayed type hypersensitivity response, and stimulation of the Th2 cell response, which results in elevated antibody production. The JES3-19F1 antibody reacts with human and viral interleukin-10 (IL-10). The JES3-19F1 antibody can neutralize the bioactivity of natural or recombinant IL-10.

Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
COS-expressed recombinant human IL-10
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

ELISA or ELISPOT Capture1-4: The Purified JES3-19F1 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated JES3-12G8 antibody (Cat. Nos. 501502 & 501503) as the detecting antibody. The Ultra-LEAF™ Purified antibody is suggested for ELISPOT capture. For use as an ELISPOT capture antibody, a concentration range of 4.0 - 8.0 µg/ml is recommended.
Flow Cytometry: The fluorochrome-labeled JES3-19F1 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-10 -producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.biolegend.com and click on the support section.
Neutralization1,2: The Ultra-LEAF™ Purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of human IL-10 bioactivity (Cat. Nos. 506814 & 506815).
Additional reported applications (for the relevant formats) include: Western blotting, immunohistochemical staining5,6 of paraformaldehyde-fixed, saponin-treated frozen tissue sections, and immunocytochemistry.
Note: For testing human IL-10 in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 430601 to 430606) are specially developed and recommended.

Application References

(PubMed link indicates BioLegend citation)
  1. Abrams J, et al. 1992. Immunol. Rev. 127:5.
  2. Gotlieb W, et al. 1992. Cytokine 4:385.
  3. Yssel H, et al. 1992. J. Immunol. 149:2378.
  4. Burdin N, et al. 1993. J. Exp. Med. 177:295.
  5. Andersson U, et al. 1999. Detection and quantification of gene expression. New York: Springer-Verlag.
  6. Andersson J, et al. 1994. Immunology 83:16.
Product Citations
  1. Yu ED, et al. 2022. Cell Rep Methods. 2:100350. PubMed
  2. Zhang B, et al. 2023. Signal Transduct Target Ther. 8:28. PubMed
  3. Yang R, et al. 2020. Cell. 183(7):1826-1847.e31. PubMed
  4. Rydyznski Moderbacher C, et al. 2020. Cell. 183(4):996-1012.e19. PubMed
  5. Riese P, et al. 2022. Nat Commun. 13:6894. PubMed
RRID
AB_2632782 (BioLegend Cat. No. 506811)
AB_2632782 (BioLegend Cat. No. 506812)

Antigen Details

Structure
Acid-labile cytokine; dimer; 35-40 kD (Mammalian)
Bioactivity
Inhibit IFN-γ, TNF-β, IL-2 production by TH1 clones; inhibits macrophage-mediated IL-1, IL-6, TNF-α synthesis; suppress delayed type hypersensitivity response; stimulate TH2 cell response; mast cell proliferation in
Cell Sources
Activated CD8+ and CD4+ T cells, activated monocytes, mast cells, Ly-1 B (mouse)
Cell Targets
T cells, B cells, mast cells, macrophages
Receptors
IL-10R (CDw210)
Cell Type
Tregs
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. de Waal-Malefyt R, et al. 1992. Curr. Opin. Immunol. 4:314.
3. Howard M, et al. 1992. Immunol. Today. 13:198.
4. Quesniaux V. 1992. Research Immunol. 143:385.

Regulation
Production inhibited by IL-4, IL-10
Gene ID
3586 View all products for this Gene ID
UniProt
View information about IL-10 on UniProt.org

Other Formats

View All IL-10 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-human IL-10 JES3-19F1 ICFC
PE anti-human IL-10 JES3-19F1 ICFC
Purified anti-human IL-10 JES3-19F1 ELISA Capture,IHC-F,ICC,WB
PE/Dazzle™ 594 anti-human IL-10 JES3-19F1 ICFC
Ultra-LEAF™ Purified anti-human IL-10 JES3-19F1 ELISA Capture,IHC-F,ICC,WB
Go To Top Version: 1    Revision Date: 11/15/2016

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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