PE/Dazzle™ 594 anti-mouse CD124 (IL-4Rα) Antibody

Pricing & Availability
Clone
I015F8 (See other available formats)
Regulatory Status
RUO
Other Names
IL-4R
Isotype
Rat IgG2b, κ
I015F8_PEDazzle-594_CD124_Antibody_2_021022_updated
C57BL/6 mouse splenocytes were stained with anti-mouse/human CD45R/B220 APC and anti-mouse CD124 (clone I015F8) PE/Dazzle™ 594 (left) or rat IgG2b, κ PE/Dazzle™ 594 isotype control (right).
  • I015F8_PEDazzle-594_CD124_Antibody_2_021022_updated
    C57BL/6 mouse splenocytes were stained with anti-mouse/human CD45R/B220 APC and anti-mouse CD124 (clone I015F8) PE/Dazzle™ 594 (left) or rat IgG2b, κ PE/Dazzle™ 594 isotype control (right).
Compare all formats See PE/Dazzle™ 594 spectral data
Cat # Size Price Quantity Check Availability
144813 25 µg $194.00
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144814 100 µg $434.00
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Description

CD124, a subunit of IL-4R, is a 140 kD transmembrane glycoprotein. It associates with either the common γ-chain (CD132) to form the type I IL-4R complex, which specifically binds IL-4, or with IL-13Ra1 to form the type II IL-4R heterodimeric complex, which binds and transduces signals from either IL-4 or IL-13.  A truncated form of IL-4Rα exists in soluble form in biological fluids. CD124 is expressed on mouse late precursors and mature B cells, T cells, monocytes, and has low expression on granulocytes.  In B cells, CD124 can bind IL-4 and IL-13 and regulate IgE antibody production. In T cells, the type I IL-4R (IL-4R/gC) is mostly responsible for Th2 cell expansion by mediating IL-4-dependent activation of the transcription factors in hematopoietic cells. The type II IL-4R (IL-4R/IL-13Ra1) is the main route for nonhematopoietic cell responses to IL-4 or IL-13.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant Mouse IL4Rα encoding the extracellular domain (amino acid residues 26 - 233) was fused to the carboxy-terminal Fc region of human IgG1 via a linker peptide.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC – Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID
AB_2910298 (BioLegend Cat. No. 144813)
AB_2910298 (BioLegend Cat. No. 144814)

Antigen Details

Structure
140 kD type I transmembrane glycoprotein, associates with common γ chain or IL-13 receptor alpha-1 subunit
Distribution

B cells, T cells, monocytes, and low levels on granulocytes.

Function
Regulates IgE antibody production in B cells, Th2 cell expansion, and IL-4 or IL-13 induced response in non-hematopoietic cells
Interaction
STAT6
Ligand/Receptor
IL-4, IL-13
Cell Type
B cells, Granulocytes, Monocytes, T cells
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Signal Transduction, Transcription Factors
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Kashiwada M, et al. 2001. J. Immunol. 167:6382.
2. Gilmour J, et al. 2008. J. Immunol. 124:437.
3. Hage T, et al. 1999. Cell 97:271.

Gene ID
16190 View all products for this Gene ID
UniProt
View information about CD124 on UniProt.org
Go To Top Version: 1    Revision Date: 02/09/2022

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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