PE/Dazzle™ 594 anti-mouse CD62L Antibody

Pricing & Availability
Clone
W18021D (See other available formats)
Regulatory Status
RUO
Other Names
L-selectin
Isotype
Rat IgG2a, κ
W18021D_PEDazzle594_CD62L_Antibody_053024
C57BL/6 mouse splenocytes were stained with anti-mouse CD3ε (clone 145-2c11) APC and anti-mouse CD62L (clone W18021D) PE/Dazzle™ 594 (left) or rat IgG2a, κ PE/Dazzle™ 594 isotype control (right).
  • W18021D_PEDazzle594_CD62L_Antibody_053024
    C57BL/6 mouse splenocytes were stained with anti-mouse CD3ε (clone 145-2c11) APC and anti-mouse CD62L (clone W18021D) PE/Dazzle™ 594 (left) or rat IgG2a, κ PE/Dazzle™ 594 isotype control (right).
Compare all formats See PE/Dazzle™ 594 spectral data
Cat # Size Price Quantity Check Availability
161215 25 µg $145.00
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161216 100 µg $330.00
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Description

CD62L is a 74-95 kD glycoprotein also known as L-selectin. It is a member of the selectin family and is expressed on the majority of B and naïve T cells, a subset of memory T cells, monocytes, granulocytes, most thymocytes, and a subset of NK cells. CD62L is important in lymphocyte homing to high endothelial venules (HEV) in peripheral lymph nodes and leukocyte "rolling" on activated endothelium. CD62L also contributes to neutrophil emigration at inflammatory sites. CD62L is rapidly shed from lymphocytes and neutrophils upon cellular activation and the expression levels of CD62L (in conjunction with other markers) have been used to distinguish naïve, effector, and memory T cells. CD62L has been reported to interact with CD34, GlyCAM-1, and MAdCAM-1.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse CD62L recombinant protein (39-332)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID
AB_3662200 (BioLegend Cat. No. 161215)
AB_3662200 (BioLegend Cat. No. 161216)

Antigen Details

Structure
CD62L is a type I transmembrane glycoprotein with 74-95 kD.
Distribution

CD62L is expressed on the majority of B and naïve T cells, a subset of memory T cells, monocytes, granulocytes, most thymocytes, and a subset of NK cells.

Function
CD62L plays roles in lymphocyte homing to lymphoid organs and inflammatory leukocyte trafficking.
Ligand/Receptor
CD34, GlyCAM-1, MAdCAM-1
Cell Type
B cells, Granulocytes, Monocytes, Neutrophils, NK cells, T cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Rosen S. 2004. Annu. Rev. Immunol. 22:129
2. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.

Gene ID
20343 View all products for this Gene ID
UniProt
View information about CD62L on UniProt.org
Go To Top Version: 1    Revision Date: 05/30/2024

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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