PE/Dazzle™ 594 anti-mouse IL-10 Antibody

Pricing & Availability
Clone
JES5-16E3 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-10, Cytokine synthesis inhibitory factor (CSIF), B cell derived T cell growth factor (B-TCGF)
Isotype
Rat IgG2b, κ
JES5-16E3_PEDZL594_IL-10_Antibody_ICFC_1_102715
PMA+ionomycin-stimulated (six hours in the presence of monensin) Th2-polarized BALB/c splenocytes were stained with CD3 Alexa Fluor® 488, fixed, permeabilized, and then stained with IL-10 (clone JES5-16E3) PE/Dazzle™ 594 (top) or rat IgG2b, κ PE/Dazzle™ 594 isotype control (bottom).
  • JES5-16E3_PEDZL594_IL-10_Antibody_ICFC_1_102715
    PMA+ionomycin-stimulated (six hours in the presence of monensin) Th2-polarized BALB/c splenocytes were stained with CD3 Alexa Fluor® 488, fixed, permeabilized, and then stained with IL-10 (clone JES5-16E3) PE/Dazzle™ 594 (top) or rat IgG2b, κ PE/Dazzle™ 594 isotype control (bottom).
  • JES5-16E3_PEDZL594_IL-10_Antibody_ICFC_2_102715
Compare all formats See PE/Dazzle™ 594 spectral data
Cat # Size Price Quantity Check Availability
505033 25 µg $136.00
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505034 100 µg $335.00
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Description

IL-10 was originally described as Cytokine Synthesis Inhibitory Factor (CSIF) by virtue of its ability to inhibit cytokine production by Th1 clones. IL-10 shares over 80% sequence homology with the Epstein-Barr virus protein BCRFI. IL-10 inhibits IFN-γ, TNF-β, and IL-2 production by Th1 clones; inhibits macrophage-mediated IL-1, IL-6, and TNF-α synthesis; suppresses the delayed type hypersensitivity response; stimulates Th2 cell response (which results in elevated antibody production); and promotes mast cell proliferation in combination with IL-4.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
E. coli-expressed, recombinant mouse IL-10
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

ELISA or ELISPOT Detection1,9,11: The biotinylated JES5-16E3 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified JES5-2A5 antibody (Cat. Nos. 504902 & 504904) as the capture antibody.
ELISA Capture: The purified JES5-16E3 antibody is useful as the capture antibody in a sandwich ELISA when used in conjunction with the biotinylated JES5-2A5 antibody (Cat. Nos. 504905 & 504906) as the detection antibody and recombinant mouse IL-10 (Cat. No. 575809) as the standard.
Neutralization14: The Ultra-LEAF™ purified JES5-16E3 antibody can neutralize the bioactivity of natural or recombinant IL-10.
Flow Cytometry3: The fluorochrome-labeled JES5-16E3 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-10-producing cells within mixed cell populations.
Additional reported applications (for relevant formats) include: immunohistochemistry3.

Application References

(PubMed link indicates BioLegend citation)
  1. Simkin G, et al. 2000. J. Immunol. 164:2457.
  2. Kitagaki K, et al. 2002. Clin. Diagn. Lab Immunol. 9:1260.
  3. Khanna A, et al. 2000. J. Immunol. 164:1346.
  4. Sander B, et al. 1993. J. Immunol. Methods 166:201.
  5. Litton M, et al. 1994. J. Immunol. Methods 175:47.
  6. Andersson U, et al. 1999. Detection and qunatification of gene expression. New York:Springer-Verlag.
  7. Finkelman F, et al. 2003. Curr. Prot. Immunol. John Wiley & Sons New York. Unit 6.28.
  8. Wang W, et al. 2004. FASEB J. 18:1043.
  9. Brummel R and Lenert P. 2005. J. Immunol. 174:2429.
  10. Lawson BR, et al. 2007. J. Immunol. 178:5366.
  11. Xu G, et al. 2007. J. Immunol. 179:5358. PubMed
  12. Brummel R, et al. 2005. J. Immunol.174:2429. PubMed
  13. Kang YJ, et al. 2007. Stem Cells 25:1814. PubMed
  14. Seo N, et al. 2001. Immunology. 103:449. (Neut)
Product Citations
  1. Henneberg LT, et al. 2023. Nat Chem Biol. 1513:19. PubMed
  2. Marques RM, et al. 2021. Cell Death Differ. 28:3140. PubMed
  3. Palathingal Bava E, et al. 2022. JCI Insight. 7:. PubMed
RRID
AB_2566328 (BioLegend Cat. No. 505033)
AB_2566328 (BioLegend Cat. No. 505034)

Antigen Details

Structure
Acid-labile cytokine, dimer, 17-21 kD (Mammalian)
Cell Sources
Activated CD8+ T cells, Th0, Th2 subset of CD4+ T cells, Ly-1+ B cells, monocytes, macrophages, keratinocytes
Cell Targets
T cells, B cells, mast cells, macrophages
Receptors
IL-10R (CDw210)
Cell Type
Tregs
Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. de Waal-Malefy R, et al. 1992. Curr. Opin. Immunol. 4:314.
3. Howard M, et al. 1992. Immunol. Today 13:198.
4. Quesniaux V. 1992. Res. Immunol. 143:385.
5. Norton SK, et al. 2008. J. Immunol. 180:2848.

Regulation
Downregulated by IL-4, IL-10
Gene ID
16153 View all products for this Gene ID
UniProt
View information about IL-10 on UniProt.org

Other Formats

View All IL-10 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse IL-10 JES5-16E3 ICFC
Biotin anti-mouse IL-10 JES5-16E3 ELISA Detection,ELISPOT Detection,ICFC
FITC anti-mouse IL-10 JES5-16E3 ICFC
PE anti-mouse IL-10 JES5-16E3 ICFC
Purified anti-mouse IL-10 JES5-16E3 ELISA Capture,CyTOF®,IHC
Alexa Fluor® 647 anti-mouse IL-10 JES5-16E3 ICFC
PE/Cyanine7 anti-mouse IL-10 JES5-16E3 ICFC
Alexa Fluor® 488 anti-mouse IL-10 JES5-16E3 ICFC
Brilliant Violet 421™ anti-mouse IL-10 JES5-16E3 ICFC
Pacific Blue™ anti-mouse IL-10 JES5-16E3 ICFC
PerCP/Cyanine5.5 anti-mouse IL-10 JES5-16E3 ICFC
Purified anti-mouse IL-10 (Maxpar® Ready) JES5-16E3 ELISA Capture,CyTOF®
Brilliant Violet 605™ anti-mouse IL-10 JES5-16E3 ICFC
PE/Dazzle™ 594 anti-mouse IL-10 JES5-16E3 ICFC
APC/Cyanine7 anti-mouse IL-10 JES5-16E3 ICFC
Ultra-LEAF™ Purified anti-mouse IL-10 JES5-16E3 ELISA Capture,CyTOF®,ELISPOT Capture,Neut,ICFC,IHC
Brilliant Violet 650™ anti-mouse IL-10 JES5-16E3 ICFC
Brilliant Violet 711™ anti-mouse IL-10 JES5-16E3 ICFC
Go To Top Version: 1    Revision Date: 10/28/2015

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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