PE/Dazzle™ 594 anti-rat CD45 Antibody

Pricing & Availability
Clone
OX-1 (See other available formats)
Regulatory Status
RUO
Other Names
Leukocyte common antigen, LCA, T200, Ly-5
Isotype
Mouse IgG1, κ
OX-1_PEDZL594_CD45_Antibody_102517
LOU rat splenocytes were stained with CD45 (clone OX-1) PE/Dazzle™ 594 (filled histogram) or mouse IgG1, κ PE/Dazzle™ 594 isotype control (open histogram).
  • OX-1_PEDZL594_CD45_Antibody_102517
    LOU rat splenocytes were stained with CD45 (clone OX-1) PE/Dazzle™ 594 (filled histogram) or mouse IgG1, κ PE/Dazzle™ 594 isotype control (open histogram).
Compare all formats See PE/Dazzle™ 594 spectral data
Cat # Size Price Quantity Check Availability
202223 25 µg $147.00
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202224 100 µg $382.00
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Description

CD45 is a 180-220 kD protein also known as leukocyte common antigen (LCA). It is a protein tyrosine phosphatase with multiple isoforms differing as a result of alternative splicing of the extracellular domain and glycosylation. CD45 is expressed on all hematopoietic cells except erythrocytes and platelets; isoform expression depends on cell type, activation state, and cell maturation. CD45 functions in signal transduction through T and B cell antigen receptors. CD45 has been shown to interact with various proteins including galectin-1, CD2, CD3, and CD4. The OX-1 antibody has been shown to partially inhibit NK cell-mediated lysis of syngeneic tumor cells in vitro.

Technical data sheet

Product Details

Verified Reactivity
Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Enriched glycoprotein fraction from Wistar rat thymocytes
Formulation
The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 under optimal conditions. The solution is free of unconjugated PE/Dazzle™ 594 and unconjugated antibody.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. 

* PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunofluorescence microscopy (acetone fixed, ammonium-thiocyanate separated epidermal sheets)2, immunohistochemistry of acetone3-, isopentane-4, PLP5-, and n-hexane6 fixed frozen sections and paraffin-embedded sections7,8, and partial inhibition of NK cell lysis of syngeneic tumor cell lines1.

Application References

(PubMed link indicates BioLegend citation)
  1. Giezeman-Smits KM, et al. 1999. J. Immunol. 163:71. (IP)
  2. Elbe A, et al. 1994. J. Invest. Dermatol. 102:74. (IF)
  3. Kouwenhoven E, et al. 2001. Kidney Int. 59:1142. (IHC)
  4. Martin A, et al. 1995 Clin. Exp. Immunol. 22:283. (IHC)
  5. Sayegh MH, et al. 1995 J. Exp. Med. 181:186. (IHC)
  6. Morioka Y, et al. 2000. Kidney Int. 60:2192. (IHC)
  7. Ng YY, et al. 2005. Kidney Int. 94:S83. (IHC-P)
  8. Huang XR, et al. 2003. JASN. 14:1738. (IHC-P)
Product Citations
  1. Gwin MS, et al. 2023. Am J Physiol Lung Cell Mol Physiol. L174:325. PubMed
  2. Yin M, et al. 2019. Int J Mol Med. 43:2086. PubMed
  3. Muñoz NM, et al. 2021. J Immunother Cancer. 9:. PubMed
  4. Muñoz NM, et al. 2020. Commun Biol. 3:783. PubMed
RRID
AB_2721705 (BioLegend Cat. No. 202223)
AB_2721705 (BioLegend Cat. No. 202224)

Antigen Details

Structure
Protein tyrosine phosphatase, type I transmembrane protein, 180-220 kD (multiple isoforms and differential glycosylation)
Distribution

All hematopoietic cells except erythrocytes and platelets

Function
T cell receptor and B cell receptor signal transduction
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Sunderland CA, et al. 1979. Eur. J. Immunol. 9:155.
2. Woolett GR, et al. 1985. Eur. J. Immunol.. 15:168.

Gene ID
24699 View all products for this Gene ID
UniProt
View information about CD45 on UniProt.org
Go To Top Version: 0    Revision Date: 10/26/2017

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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