- Clone
- 15F9 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Inducible COStimulatory molecule, H4, CD278
- Isotype
- Syrian Hamster IgG
-
C57BL/6 mouse splenocytes were cultured with ConA/IL-2 for three days. Cells were stained with anti-mouse CD278 (ICOS) (clone 15F9) PE/Fire™ 640 (filled histogram) or left unstained (open histogram).
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 107713 | 25 µg | $94.00 | |||
| 107714 | 100 µg | $242.00 | |||
CD278 is a 47-57 kD protein, also known as inducible costimulatory molecule (ICOS), or H4. This protein is homologous to the CD28/CTLA-4 proteins. It forms homodimers and is expressed on activated T cells and a subset of thymocytes. It is able to costimulate T cells proliferation. In addition, ICOS has been shown to be involved in humoral immune responses (B cell germinal center formation). The ICOS ligand is B7-H2. ICOS stimulation has been shown to potentiate TCR-mediated IL-4 and IL-10 production and has been proposed to play a role in Th2 cell development. It has also been shown to be involved in airway tolerance and the downregulation of pulmonary inflammation.
Product Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Syrian Hamster
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE/Fire™ 640 under optimal conditions.
- Concentration
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PE/Fire™ 640 has a maximum excitation of 566 nm and a maximum emission of 639 nm.
- Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
-
Additional reported applications (for the relevant formats) include: immunohistochemical staining2 of acetone-fixed frozen sections.
-
Application References
(PubMed link indicates BioLegend citation) -
- Dong C, et al. 2001. Nature 409(6816):97.
- Walker LSK, et al. 2003. J. Immunol. 170:91.
- RRID
-
AB_2924445 (BioLegend Cat. No. 107713)
AB_2924445 (BioLegend Cat. No. 107714)
Antigen Details
- Structure
- CD28/CTLA-4, 47-57kD
- Distribution
-
Activated T cells, subset of thymocytes
- Function
- Costimulates T cell activation, proliferation, humoral immune response
- Ligand/Receptor
- B7h/B7RP-1/GL-50
- Cell Type
- T cells, Thymocytes, Tregs
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
- Rudd CE, et al. 2003. Nat. Rev. Immunol. 3:544.
- McAdam AJ, et al. 2000. J. Immunol. 165:5035.
- Mak TW, et al. 2003. Nat. Immunol. 4:765
- Gene ID
- 54167 View all products for this Gene ID
- UniProt
- View information about CD278 on UniProt.org
Other Formats
View All CD278 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| PE anti-mouse CD278 (ICOS) | 15F9 | FC |
| PE/Cyanine5 anti-mouse CD278 (ICOS) | 15F9 | FC |
| APC anti-mouse CD278 (ICOS) | 15F9 | FC |
| PE/Fire™ 640 anti-mouse CD278 (ICOS) | 15F9 | FC |
| PE/Fire™ 700 anti-mouse CD278 (ICOS) | 15F9 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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PE anti-mouse CD278 (ICOS)
Con A-stimulated (3 days) BALB/c splenocytes stained with 15... -
PE/Cyanine5 anti-mouse CD278 (ICOS)
ConA-stimulated C57BL/6 mouse splenocytes stained with 15F9 ... -
APC anti-mouse CD278 (ICOS)
C57BL/6 mouse splenocytes were cultured with ConA/IL-2 for t... -
PE/Fire™ 640 anti-mouse CD278 (ICOS)
C57BL/6 mouse splenocytes were cultured with ConA/IL-2 for t... -
PE/Fire™ 700 anti-mouse CD278 (ICOS)
C57BL/6 mouse splenocytes were cultured with ConA/IL-2 for t...