PE/Fire™ 700 anti-mouse CD272 (BTLA) Antibody

Pricing & Availability
Clone
6A6 (See other available formats)
Regulatory Status
RUO
Other Names
BTLA, B and T lymphocyte attenuator
Isotype
Armenian Hamster IgG
6A6_PEFire-700_CD272_Antibody_032922
C57BL/6 mouse splenocytes were stained with anti-mouse/human CD45R/B220 FITC and anti-mouse CD272 (clone 6A6) PE/Fire™ 700 (left) or stained with anti-mouse/human CD45R/B220 FITC only (right).
  • 6A6_PEFire-700_CD272_Antibody_032922
    C57BL/6 mouse splenocytes were stained with anti-mouse/human CD45R/B220 FITC and anti-mouse CD272 (clone 6A6) PE/Fire™ 700 (left) or stained with anti-mouse/human CD45R/B220 FITC only (right).
Compare all formats See PE/Fire™ 700 spectral data
Cat # Size Price Quantity Check Availability
139127 25 µg $143.00
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139128 100 µg $319.00
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Description

CD272, also known as B and T lymphocyte attenuator (BTLA), is an Ig superfamily co-inhitory receptor with structural similarity to programmed cell death 1 (PD-1) and CTLA-4.  BTLA is expressed on B cells, T cells, macrophages, dendritic cells, NKT cells, and NK cells. Engagement of BTLA by its ligand herpes virus entry mediator (HVEM) is critical for negatively regulating immune response.  The absence of BTLA with HVEM inhibitory interactions leads to increased experimental autoimmune encephalomyelitis severity, enhanced rejection of partially mismatched allografts, an increased CD8+ memory T cell population, increased severity of colitis, and reduced effectiveness of T regulatory cells.  BTLA plays an important role in the induction of peripheral tolerance of both CD4+ and CD8+ T cells in vivo.  Tolerant T cells have significantly higher expression of BTLA compared with effectors and naïve T cells.  BTLA may cooperate with CTLA-4 and PD-1 to control T cell tolerance and autoimmunity.  It was reported that BTLA may regulate T cell function by binding to B7-H4, but further studies are needed to confirm.  The existence of three distinct BTLA alleles has been reported.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
C57BL/6 BTLA Ig domain protein in CFA
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Fire™ 700 under optimal conditions.
Concentration
0.2 mg/mL
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PE/Fire™ 700 has a maximum excitation of 565 nm and a maximum emission of 695 nm.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for relevant formats) include: immunoprecipitation1,2 and blocking3,5 of OT-I T cell responses in vitro and incidence of cerebral malaria in vivo caused by P. berghei ANKA (PbA) infection. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 139104). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 139117 - 139122) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).

Clone 6A6 antibody reacts only with the C57BL/6 allele of BTLA but not the BALB/c allele of BTLA.

Application References

(PubMed link indicates BioLegend citation)
  1. Hurchla MA, et al. 2005. J. Immunol. 174:337. (IP FC)
  2. Sedy JR, et al. 2005. Nat. Immunol. 6:90. (IP)
  3. Lepenies B, et al. 2007. J. Immunol. 179:4093. (Block)
  4. Tao R, et al. 2005. J. Immunol. 175:5774. (FC)
  5. Albring J, et al. 2010. J. Exp. Med. 207:2551. (Block)
RRID
AB_2924471 (BioLegend Cat. No. 139127)
AB_2924471 (BioLegend Cat. No. 139128)

Antigen Details

Structure
An Ig superfamily co-inhibitory receptor with structural similarity to programmed cell death 1 (PD-1) and CTLA-4.
Distribution

BTLA is expressed on a wide number of lymphocytes in mice. It is most highly expressed on B cells, followed by CD4+ T cells, lower expression on CD8+ T cells, macrophages, dendritic cells, NKT cells, and NK cells.

Function
BTLA functions as a negative regulator of T cell activation and proliferation, attenuate B cell proliferation upon associating with its known ligand, herpes virus entry mediator (HVEM).
Ligand/Receptor
HVEM.
Cell Type
B cells, Dendritic cells, Lymphocytes, NKT cells, T cells
Biology Area
Immunology, Inhibitory Molecules
Molecular Family
CD Molecules
Antigen References

1. Liu X, et al. 2009. J Immunol. 182:4516.
2. Miller ML, et al. 2009. J Immunol. 183:32.
3. Sun Y, et al. 2009. J Immunol. 183:1946.
4. Vendel AC, et al. 2009. J Immunol. 182:1509.
5. Watanabe N, et al. 2003. Nat Immunol. 4:670.

Gene ID
208154 View all products for this Gene ID
UniProt
View information about CD272 on UniProt.org
Go To Top Version: 1    Revision Date: 03/29/2022

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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