PE/Fire™ 744 anti-mouse CD64 (FcγRI) Antibody

Pricing & Availability
Clone
S18017D (See other available formats)
Regulatory Status
RUO
Other Names
FcRI
Isotype
Rat IgG2a, κ
S18017D_PEFire744_CD64_060324
C57BL/6 mouse bone marrow cells were stained with anti-mouse/human CD11b (clone M1/70) APC and anti-mouse CD64 (FcγRI) (clone S18017D) PE/Fire™ 744 (left) or rat IgG2a, κ PE/Fire™ 744 isotype control (right). Data shown was gated on myeloid cells.
  • S18017D_PEFire744_CD64_060324
    C57BL/6 mouse bone marrow cells were stained with anti-mouse/human CD11b (clone M1/70) APC and anti-mouse CD64 (FcγRI) (clone S18017D) PE/Fire™ 744 (left) or rat IgG2a, κ PE/Fire™ 744 isotype control (right). Data shown was gated on myeloid cells.
Compare all formats See PE/Fire™ 744 spectral data
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161009 25 µg $176.00
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161010 100 µg $382.00
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Description

CD64 is a 72 kD single chain type I glycoprotein also known as FcγRI and FcRI. CD64 is a member of the immunoglobulin superfamily and is expressed on monocytes/macrophages, dendritic cells, and mast cells. The expression can be upregulated by IFN-γ stimulation. CD64 binds IgG immune complex. It plays a role in antigen capture, phagocytosis of IgG/antigen complexes, and antibody-dependent cellular cytotoxicity (ADCC).

Technical data sheet

Product Details

Verified Reactivity
Mouse, Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant mouse FcγRI/CD64 (Cat. No. 773806)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Fire™ 744 under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.06 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

True-Stain Monocyte Blocker™ (Cat No. 426102) is recommended to minimize non-specific staining of PE/Fire™ 744 on monocytes and macrophages.

* PE/Fire™ 744 has a maximum excitation of 565 nm and a maximum emission of 744 nm.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID
AB_3106100 (BioLegend Cat. No. 161009)
AB_3106100 (BioLegend Cat. No. 161010)

Antigen Details

Structure
Ig superfamily, type I glycoprotein, 72 kD
Distribution

Monocytes, macrophages, mast cells, dendritic cells

Function
Phagocytosis, ADCC
Ligand/Receptor
IgG
Antigen References

1. Schyns J, et al. 2019. Nat. Commun. 3;10(1):3964
2. Langlet C, et al. 2012. J. Immunol. 188 (4) 1751-1760.

Gene ID
14129 View all products for this Gene ID
UniProt
View information about CD64 on UniProt.org
Go To Top Version: 1    Revision Date: 03/11/2024

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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