PerCP/Cyanine5.5 anti-human CD25 Antibody, CD25, M-A251

Pricing & Availability

Analyte Specific Reagent. Analytical and performance characteristics are not established.

Clone: M-A251
Workshop: IV A053
Other Names: IL-2 receptor α chain, Low affinity IL-2R, IL-2Rα chain
Isotype: Mouse IgG1, κ

Typical results from human peripheral blood lymphocytes stained either with M-A251 PerCP/Cyanine5.5 used at 5 µL/test (filled histogram) or with an isotype control (open histogram).

Compare all formats See PerCP/Cyanine5.5 spectral data

Cat #

Size

Price

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985804

500 µL

$588.00

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Description

CD25 is a 55 kD type I transmembrane glycoprotein also known as low affinity IL-2 receptor α chain or Tac. It is expressed on progenitor lymphocytes, activated T and B cells, and activated monocytes/macrophages. CD25 is also expressed on a subset of non-stimulated CD4⁺ T cells termed T regulatory cells. Soluble CD25/IL-2Rα is produced as a consequence of lymphocyte stimulation and is found in biological fluids following inflammatory responses. CD25 associates with IL-2 receptor β (CD122) and common γ (CD132) chains to form a high affinity IL-2R complex.

Technical data sheet

Product Details

Reactivity

Human

Formulation

Phosphate-buffered solution, pH 7.2, containing True-Stain Monocyte Blocker™, 0.09% sodium azide, 0.2% (w/v) BSA (origin USA), and a stabilizer.

Preparation

The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.

Concentration

100 µg/mL

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

Suggested for Flow Cytometry

Disclaimer

WARNINGS AND PRECAUTIONS

Use appropriate personal protective equipment and safety practices per universal precautions when working with this reagent. Refer to the reagent safety data sheet.
This antibody contains sodium azide. Follow federal, state and local regulations to dispose of this reagent. Sodium azide build-up in metal wastepipes may lead to explosive conditions; if disposing of reagent down wastepipes, flush with water after disposal.
All specimens, samples and any material coming in contact with them should be considered potentially infectious and should be disposed of with proper precautions and in accordance with federal, state and local regulations.
Do not use this reagent beyond the expiration date stated on the label.
Do not use this reagent if it appears cloudy or if there is any change in the appearance of the reagent as these may be an indication of possible deterioration.
Avoid prolonged exposure of the reagent or stained cells to light.

Antigen Details

Antigen References: 1. Knapp W, et al. 1989. Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press.
2. Schlossman S, et al. 1995. Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press.
3. Barclay N, et al. 1997. The Leukocyte Antigen FactsBook. Academic Press Inc.
4. Taniguchi T and Minami Y. et al. 1993. Cell 73:5.
5. Waldmann T. 1991. J. Biol. Chem. 266:2681.

Go To Top Version: 1 Revision Date: 07/15/2021

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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