PerCP/Cyanine5.5 anti-human CD4 Antibody

Pricing & Availability
Clone
SK3 (See other available formats)
Regulatory Status
RUO
Other Names
T4, Leu3a
Isotype
Mouse IgG1, κ
SK3_PerCPCyanine55_CD4_Antibody_101618
Human peripheral blood lymphocytes were stained with anti-human CD3 APC and anti-human CD4 (clone SK3) PerCP/Cyanine5.5 (left), or mouse IgG1, κ PerCP/Cyanine5.5 isotype control (right).
  • SK3_PerCPCyanine55_CD4_Antibody_101618
    Human peripheral blood lymphocytes were stained with anti-human CD3 APC and anti-human CD4 (clone SK3) PerCP/Cyanine5.5 (left), or mouse IgG1, κ PerCP/Cyanine5.5 isotype control (right).
Compare all formats See PerCP/Cyanine5.5 spectral data
Cat # Size Price Quantity Check Availability
344607 25 tests $191.00
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344608 100 tests $401.00
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Description

CD4, also known as T4, is a 55 kD single-chain type I transmembrane glycoprotein expressed on most thymocytes, a subset of T cells, and monocytes/macrophages. CD4, a member of the Ig superfamily, recognizes antigens associated with MHC class II molecules and participates in cell-cell interactions, thymic differentiation, and signal transduction. CD4 acts as a primary receptor for HIV, binding to HIV gp120. CD4 has also been shown to interact with IL-16.

Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Evans RL, et al. 1981. Immunol. 78:544
  2. Arno A et al. 1999. J. Infect. Dis. 180:56
  3. Muech M, et al. 1997. Blood 89:1364
  4. Wang L, et al. 2012. Cytometry A. 81:567. PubMed
Product Citations
  1. Wang J, et al. 2022. J Leukoc Biol. 112:1567. PubMed
  2. Manukyan G, et al. 2020. Auto Immun Highlights. 11:5. PubMed
  3. Cao M, et al. 2020. Aging Dis. 1.479861111. PubMed
  4. Matzen SMH, et al. 2018. Health Sci Rep. 1:e90. PubMed
  5. Klichinsky M, et al. 2020. Nat Biotechnol. 38:947. PubMed
  6. Yamaguchi A, et al. 2021. J Thorac Dis. 13:5430. PubMed
  7. Sung BY, et al. 2022. J Clin Invest. 132:. PubMed
  8. Atwany NZ, et al. 2020. Biology (Basel). 9:00. PubMed
  9. Schoutrop E, et al. 2022. Oncoimmunology. 11:2093426. PubMed
  10. Lozano-Rodríguez R, et al. 2022. Cell Rep. 38:110235. PubMed
  11. Lima J, et al. 2016. Reprod Sci. 10.1177/1933719116653680. PubMed
  12. Libera J, et al. 2020. Front Immunol. 11:567472. PubMed
  13. Shuwa HA, et al. 2021. Med. 2(6):720-735.e4. PubMed
  14. Roudko V, et al. 2020. Cell. 183(6):1634-1649.e17. PubMed
  15. Gustafson CE, et al. 2020. JCI Insight. 5:. PubMed
  16. Faissner S, et al. 2022. Front Immunol. 13:980526. PubMed
  17. Nasrallah R, et al. 2020. Nature. 583:447. PubMed
RRID
AB_1953235 (BioLegend Cat. No. 344607)
AB_1953235 (BioLegend Cat. No. 344608)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, 55 kD
Distribution

T cell subset, majority of thymocytes, monocytes/macrophages

Function
MHC class II co-receptor, lymphocyte adhesion, thymic differentiation, HIV receptor
Ligand/Receptor
MHC class II molecules, HIV gp120, IL-16
Cell Type
Macrophages, Monocytes, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Center D et al. 1996. Immunol. Today 17:476.
2. Gaubin M et al. 1996. Eur. J. Clin. Chem. Clin. Biochem. 34:723.

Gene ID
920 View all products for this Gene ID
UniProt
View information about CD4 on UniProt.org

Other Formats

View All CD4 Reagents Request Custom Conjugation
Description Clone Applications
PerCP anti-human CD4 SK3 FC
Purified anti-human CD4 SK3 FC,IP
FITC anti-human CD4 SK3 FC
PE anti-human CD4 SK3 FC
PerCP/Cyanine5.5 anti-human CD4 SK3 FC
Biotin anti-human CD4 SK3 FC
PE/Cyanine7 anti-human CD4 SK3 FC
APC anti-human CD4 SK3 FC
APC/Cyanine7 anti-human CD4 SK3 FC
Alexa Fluor® 488 anti-human CD4 SK3 FC
Pacific Blue™ anti-human CD4 SK3 FC
Alexa Fluor® 700 anti-human CD4 SK3 FC
Purified anti-human CD4 (Maxpar® Ready) SK3 FC,CyTOF®
PE anti-human CD4 SK3 FC
Brilliant Violet 421™ anti-human CD4 SK3 FC
Brilliant Violet 510™ anti-human CD4 SK3 FC
Alexa Fluor® 647 anti-human CD4 SK3 FC
FITC anti-human CD4 SK3 FC
APC/Fire™ 750 anti-human CD4 SK3 FC
PE/Dazzle™ 594 anti-human CD4 SK3 FC
Pacific Blue™ anti-human CD4 SK3 FC
Brilliant Violet 785™ anti-human CD4 SK3 FC
Brilliant Violet 750™ anti-human CD4 SK3 FC
Brilliant Violet 605™ anti-human CD4 SK3 FC
Brilliant Violet 711™ anti-human CD4 SK3 FC
PE/Cyanine7 anti-human CD4 SK3 FC
TotalSeq™-A0045 anti-human CD4 SK3 PG
TotalSeq™-C0045 anti-human CD4 SK3 PG
PE/Cyanine5 anti-human CD4 SK3 FC
PerCP/Cyanine5.5 anti-human CD4 SK3 FC
Spark Blue™ 550 anti-human CD4 SK3 FC
Spark NIR™ 685 anti-human CD4 SK3 FC
APC anti-human CD4 SK3 FC
KIRAVIA Blue 520™ anti-human CD4 SK3 FC
APC/Fire™ 810 anti-human CD4 SK3 FC
PE/Fire™ 640 anti-human CD4 SK3 FC
PE/Fire™ 700 anti-human CD4 SK3 FC
PerCP anti-human CD4 SK3 FC
Spark Violet™ 538 anti-human CD4 Antibody SK3 FC
Spark YG™ 581 anti-human CD4 SK3 FC
Alexa Fluor® 660 anti-human CD4 SK3 FC
Spark YG™ 593 anti-human CD4 SK3 FC
PE/Fire™ 810 anti-human CD4 Antibody SK3 FC
APC/Fire™ 750 anti-human CD4 SK3 FC
Spark Blue™ 574 anti-human CD4 SK3 FC
GMP APC anti-human CD4 SK3 FC
TotalSeq™-B0045 anti-human CD4 Antibody SK3 PG
Spark Violet™ 423 anti-human CD4 Antibody SK3 FC
GMP Pacific Blue™ anti-human CD4 SK3 FC
Spark UV™ 387 anti-human CD4 SK3 FC
GMP PE anti-human CD4 SK3 FC
GMP FITC anti-human CD4 SK3 FC
Spark Red™ 718 anti-human CD4 SK3 FC
GMP PerCP anti-human CD4 SK3 FC
GMP PE/Cyanine7 anti-human CD4 SK3 FC
Spark Violet™ 500 anti-human CD4 SK3 FC
GMP PerCP/Cyanine5.5 anti-human CD4 SK3 FC
Brilliant Violet 650™ anti-human CD4 SK3 FC
PerCP/Fire™ 806 anti-human CD4 SK3 FC
Spark Blue™ 515 anti-human CD4 SK3 FC
GMP APC/Fire™ 750 anti-human CD4 SK3 FC
Spark Violet™ 423 anti-human CD4 SK3 FC
PerCP/Fire™ 780 anti-human CD4 SK3 FC
PE/Fire™ 744 anti-human CD4 SK3 FC
Spark PLUS UV395™ anti-human CD4 SK3 FC
Spark PLUS B550™ anti-human CD4 SK3 FC
Go To Top Version: 2    Revision Date: 10/16/2018

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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