- Clone
- S21001C (See other available formats)
- Regulatory Status
- RUO
- Other Names
- L-selectin, LECAM-1, LAM-1, Leu-8, TQ-1
- Isotype
- Mouse IgG1, κ
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Human peripheral blood lymphocytes were stained with anti-human CD3 (clone UCHT1) FITC and anti-human CD62L (clone S21001C) PerCP/Cyanine5.5 (left) or mouse IgG1, κ PerCP/Cyanine5.5 isotype control (right).
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 385107 | 25 tests | $170.00 | |||
| 385108 | 100 tests | $382.00 | |||
CD62L, also referred to as L-selectin or LECAM-1, is a 74-95 kD single chain type I glycoprotein. It is expressed on most peripheral blood B cells, subsets of T and NK cells, monocytes, granulocytes, and certain hematopoietic malignant cells. CD62L binds to carbohydrates present on certain glycoforms of CD34, glycam-1, and MAdCAM-1 and with a low affinity to anionic oligosaccharide sequences related to sialylated Lewis X (sLex, CD15s) through its C-type lectin domain. CD62L is important for the homing of naïve lymphocytes to high endothelial venules in peripheral lymph nodes and Peyer's patches. It also plays a role in leukocyte rolling on activated endothelial cells.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human CD62L transfected cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm. - Excitation Laser
-
Blue Laser (488 nm)
- Application Notes
-
Clone S21001C does not block the binding of clone DREG-56 on target cells.
Clone S21001C may be more sensitive in detecting CD62L surface expression level changes. - RRID
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AB_3106118 (BioLegend Cat. No. 385107)
AB_3106118 (BioLegend Cat. No. 385108)
Antigen Details
- Structure
- Selectin, single chain glycoprotein, 74-95 kD
- Distribution
-
Majority of B cells, naïve T cells, subset of memory T and NK cells, monocytes, granulocytes, thymocytes
- Function
- Leukocyte homing, leukocyte tethering, rolling
- Ligand/Receptor
- CD34, GlyCAM, MAdCAM-1
- Cell Type
- B cells, Granulocytes, Lymphocytes, Monocytes, Neutrophils, NK cells, T cells
- Biology Area
- Cell Adhesion, Cell Biology, Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
- Kishimoto T, et al. 1990. P Natl Acad Sci USA. 87:2244.
- Kishimoto T, et al. 1991. Blood. 78:805-11.
- Gene ID
- 6402 View all products for this Gene ID
- UniProt
- View information about CD62L on UniProt.org
Other Formats
View All CD62L Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-human CD62L | S21001C | FC |
| PE anti-human CD62L | S21001C | FC |
| APC anti-human CD62L | S21001C | FC |
| PerCP/Cyanine5.5 anti-human CD62L | S21001C | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD62L
Human peripheral blood lymphocytes were stained with purifie... -
PE anti-human CD62L
Human peripheral blood lymphocytes were stained with anti-hu... -
APC anti-human CD62L
Human peripheral blood lymphocytes were stained with anti-hu... -
PerCP/Cyanine5.5 anti-human CD62L
Human peripheral blood lymphocytes were stained with anti-hu...