- Clone
- NJ-4F3-D1 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- S100 Calcium Binding Protein A4, 42A, 18A2, CAPL, FSP1, MTS1, P9KA, PEL98, Calvasculin, Metastasin, Placental calcium-binding protein
- Isotype
- Mouse IgG1, κ
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Human peripheral blood mononuclear cells were stimulated with PMA+ionomycin for 10 minutes, then fixed, permeabilized and stained with S100A4 (clone NJ-4F3-D1) PerCP/Cyanine5.5 (filled histogram) or mouse IgG1, κ PerCP/Cyanine5.5 isotype control (op
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 370009 | 25 tests | $176.00 | |||
| 370010 | 100 tests | $405.00 | |||
S100A4 is a member of the S100 family of calcium-binding proteins that contain two Ca2+-binding EF-hand motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells. S100A4 interacts with cytoskeletal proteins and enhances metastasis of several types of cancer cells. It can be secreted by unknown mechanisms, stimulating a variety of cellular responses, including angiogenesis and neuronal growth. S100A4 has been shown to be a prognostic marker in a number of human cancers. It also has a potential use as a marker for tumor metastasis and disease progression.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- cDNA
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm. - Excitation Laser
-
Blue Laser (488 nm)
- Application Notes
-
Additional reported applications (for the relevant formats) include: ELISA Detection.
-
Application References
(PubMed link indicates BioLegend citation) -
- Eisenbacher JL, et al. 2014. J. Immunol. 192:6102.
- Product Citations
-
- RRID
-
AB_2572075 (BioLegend Cat. No. 370009)
AB_2572075 (BioLegend Cat. No. 370010)
Antigen Details
- Structure
- EF-hand motif.
- Distribution
-
Widely expressed; localized in the cytoplasm and/or nucleus of a vast range of cells.
- Function
- Calcium-binding.
- Cell Type
- Neutrophils
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience, Signal Transduction
- Molecular Family
- Immune Checkpoint Receptors
- Antigen References
-
1. Schneider M, et al. 2008. J. Mol. Med. 86:507.
2. Donato R, et al. 2013. Curr. Mol. Med. 13:24.
3. Sherbet GV. 2009. Cancer Lett. 280:15. - Gene ID
- 6275 View all products for this Gene ID
- UniProt
- View information about S100A4 on UniProt.org
Other Formats
View All S100A4 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| PE anti-human S100A4 | NJ-4F3-D1 | ICFC |
| Purified anti-human S100A4 | NJ-4F3-D1 | ICFC,ELISA Detection |
| FITC anti-human S100A4 | NJ-4F3-D1 | ICFC |
| PerCP/Cyanine5.5 anti-human S100A4 | NJ-4F3-D1 | ICFC |
| APC anti-human S100A4 | NJ-4F3-D1 | ICFC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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PE anti-human S100A4
Human peripheral blood mononuclear cells were fixed and perm... -
Purified anti-human S100A4
Human peripheral blood mononuclear cells were fixed and perm... -
FITC anti-human S100A4
Human peripheral blood mononuclear cells were fixed and perm... -
PerCP/Cyanine5.5 anti-human S100A4
Human peripheral blood mononuclear cells were stimulated wit... -
APC anti-human S100A4
Human peripheral blood mononuclear cells were fixed and perm...