PerCP/Cyanine5.5 anti-mouse CD206 (MMR) Antibody

Pricing & Availability
Clone
C068C2 (See other available formats)
Regulatory Status
RUO
Other Names
MMR (macrophage mannose receptor), MR (mannose receptor), MRC1
Isotype
Rat IgG2a, κ
C068C2_PerCpCy5.5_CD206_Antibody_FC_041013
Thioglycollate-elicited BALB/c mouse peritoneal macrophages were intracellularly stained with CD206 (clone C068C2) PerCP/Cyanine5.5 (filled histogram) or rat IgG2a, κ PerCP/Cyanine5.5 isotype control (open histogram).
  • C068C2_PerCpCy5.5_CD206_Antibody_FC_041013
    Thioglycollate-elicited BALB/c mouse peritoneal macrophages were intracellularly stained with CD206 (clone C068C2) PerCP/Cyanine5.5 (filled histogram) or rat IgG2a, κ PerCP/Cyanine5.5 isotype control (open histogram).
Compare all formats See PerCP/Cyanine5.5 spectral data
Cat # Size Price Quantity Check Availability
141715 25 µg $176.00
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141716 100 µg $382.00
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Description

CD206, also known as mannose receptor (MR), is a 175 kD type I membrane protein. It is a pattern recognition receptor (PRR) belonging to the C-type lectin superfamily. MR is expressed on macrophages, dendritic cells, Langerhans cells, and hepatic or lymphatic endothelial cells. MR recognizes a range of microbial carbohydrates bearing mannose, fucose, or N-acetyl glucosamine through its C-type lectin-like carbohydrate recognition domains, sulfated carbohydrate antigens through its cysteine-rich domain, and collagens through its fibronectin type II domain. MR mediates endocytosis and phagocytosis as well as activation of macrophages and antigen presentation. It plays an important role in host defense and provides a link between innate and adaptive immunity. Recently, MR on lymphatic endothelial cells was found to be involved in leukocyte trafficking and a contributor to the metastatic behavior of cancer cells. It suggests that MR may be a potential target in controlling inflammation and cancer metastasis by targeting the lymphatic vasculature.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant mouse CD206 (MMR)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
FC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application Notes

Clone C068C2 recognizes a region similar to clone MR5D3, based on the ability of the clones to block each other. Additional reported applications (for the relevant formats) include: spatial biology (IBEX)4,5.

Application References

(PubMed link indicates BioLegend citation)
  1. Keller J, et al. 2012. Biochem Biophys Res Commun. 417:217. PubMed
  2. Ito H, et al. 2012. J Am Soc Nephrol. 23:1797. PubMed
  3. Yang X, et al. 2015. PNAS. 112:2900. PubMed
  4. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  5. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Choconta JL, et al. 2023. Immun Ageing. 20:22. PubMed
  2. Wang K, et al. 2023. Nat Commun. 14:2950. PubMed
  3. Yang C, et al. 2022. Nat Commun. 13:3426. PubMed
  4. Chen G, et al. 2021. J Mol Cell Cardiol. 159:62. PubMed
  5. Dolfi B, et al. 2022. Cell Rep. 39:110949. PubMed
  6. Gong F, et al. 2023. Adv Healthc Mater. 12:e2201771. PubMed
  7. Lin C, et al. 2020. Cancer Immunol Res. 632:8. PubMed
  8. Morfoisse F, et al. 2018. Arterioscler Thromb Vasc Biol. 38:1346. PubMed
  9. Wang S, et al. 2022. Front Immunol. 13:869061. PubMed
  10. Zhao J, et al. 2022. J Nanobiotechnology. 20:62. PubMed
  11. Yerneni SS, et al. 2021. MBio. 12:e0165721. PubMed
  12. An YA et al. 2017. eLife. 6 pii: e24071. PubMed
  13. Wang C, et al. 2021. Cell Rep. 37:110021. PubMed
  14. Ano Y, et al. 2017. J Biol Chem. 292:3720. PubMed
  15. Gao H, et al. 2021. Cells. 10:. PubMed
  16. Peng K, et al. 2017. PLoS One.. 10.1371/journal.pone.0183271. PubMed
  17. Gozgit JM, et al. 2021. Cancer Cell. :. PubMed
  18. De Santi C, et al. 2022. Mol Ther Nucleic Acids. 29:643. PubMed
  19. Uddin MJ, et al. 2022. Mucosal Immunol. 15:165. PubMed
  20. Barsoumian HB, et al. 2020. J Immunother Cancer. 8:00. PubMed
  21. Oguri Y, et al. 2020. Cell. 182(3):563-577.e20. PubMed
  22. Bronisz–Budzynska I, et al. 2019. Skelet Muscle. 9:22. PubMed
  23. Xiao Y, et al. 2022. Nat Commun. 13:758. PubMed
  24. Shigeta A, et al. 2019. Dev Cell. 48:617. PubMed
  25. Chao Y, et al. 2020. Sci Adv. 6:eaaz4204. PubMed
  26. Zhang Z, et al. 2020. Front Immunol. 11:583276. PubMed
  27. Yang C, et al. 2019. J Transl Med. 1.004166667. PubMed
  28. Calvente CJ, et al. 2019. J Clin Invest. 130:4091. PubMed
  29. Zhou Y, et al. 2022. Theranostics. 12:5488. PubMed
  30. Barsoumian HB, et al. 2022. Cancers (Basel). 14:. PubMed
  31. Li Z, et al. 2022. Evid Based Complement Alternat Med. 2022:7421265. PubMed
  32. Wang C, et al. 2020. Mucosal Immunol. 13:22. PubMed
  33. Zhang J, et al. 2020. Cell Metabolism. 31(6):1136-1153. PubMed
  34. Cortez MA, et al. 2020. Nat Commun. 3.819444444. PubMed
RRID
AB_2561991 (BioLegend Cat. No. 141715)
AB_2561991 (BioLegend Cat. No. 141716)

Antigen Details

Structure
Type I transmembrane protein, 175 kD, C-type lectin superfamily
Distribution

Macrophages, dendritic cells, Langerhans cells, liver endothelial cells

Function
Pathogen recognition, endocytosis and phagocytosis, antigen presentation
Ligand/Receptor
Antigen containing mannose, fucose, or an N-acetyl glucosamine
Cell Type
Dendritic cells, Endothelial cells, Langerhans cells, Macrophages
Biology Area
Cell Biology, Immunology, Innate Immunity, Signal Transduction
Molecular Family
CD Molecules
Antigen References

1. Wileman TE, et al. 1986. P. Natl. Acad. Sci. USA 83:2501.
2. Apostolopoulos V, et al. 2001. Curr. Mol. Med. 1:469.
3. Burgdorf S, et al. 2006. J. Immunol. 176:6770.
4. McKenzie EJ, et al. 2007. J. Immunol. 178:4975.

Gene ID
17533 View all products for this Gene ID
UniProt
View information about CD206 on UniProt.org
Go To Top Version: 3    Revision Date: 04/04/2016

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