PerCP/Cyanine5.5 anti-mouse FR4 (Folate Receptor 4) Antibody

Pricing & Availability
Clone
12A5 (See other available formats)
Regulatory Status
RUO
Other Names
Folate Receptor 4, FBP, FR δ
Isotype
Rat IgG1, κ
12A5_PerCPCyanine55_FR4_Antibody_1_101323
C57BL/6 mouse splenocytes were stained with CD3 FITC and FR4 (Folate Receptor 4, clone 12A5) PerCP/Cyanine5.5 (left) or rat IgG2b, κ PerCP/Cyanine5.5 isotype control (right).
  • 12A5_PerCPCyanine55_FR4_Antibody_1_101323
    C57BL/6 mouse splenocytes were stained with CD3 FITC and FR4 (Folate Receptor 4, clone 12A5) PerCP/Cyanine5.5 (left) or rat IgG2b, κ PerCP/Cyanine5.5 isotype control (right).
Compare all formats See PerCP/Cyanine5.5 spectral data
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125017 25 µg $159.00
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125018 100 µg $370.00
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Description

Folate receptor 4 (FR4) is a surface receptor for folic acid (Vitamin B9).It was recently reported that FR4 is highly and constitutively expressed on mouse CD4+ CD25+ natural regulatory T cells (Treg).The high expression of this surface marker on Treg can be used in combination with CD4 and CD25 to distinguish Treg from other types of T cells.Since FR4 is a surface marker,this antibody may be used in combination with anti-CD4 and/or CD25 to sort living T reg cells for functional studies.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Yamaguchi T et al. 1007:Immunity 27(1)145
RRID
AB_2721723 (BioLegend Cat. No. 125017)
AB_2721723 (BioLegend Cat. No. 125018)

Antigen Details

Structure
28 kd protein consisting of 244 amino acids. Heavily glycosylated
Distribution

Expressed on mouse thymocytes and spleen cells. Highly expressed on natural regulatory (Treg) cells

Function
Mediate calcium-independent homophlic cell-cell adhesion
Ligand/Receptor
Folic acid and its derivative
Cell Type
Tregs
Biology Area
Immunology, Innate Immunity
Antigen References

1. Yamaguchi T et al 2007. Immunity 27:145
2. Spiegelstein O et al. 2000 Gene 258:117

Gene ID
64931 View all products for this Gene ID
UniProt
View information about FR4 on UniProt.org
Go To Top Version: 1    Revision Date: 10/27/2017

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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