PerCP/Cyanine5.5 anti-mouse GM-CSF Antibody

Pricing & Availability
Clone
MP1-22E9 (See other available formats)
Regulatory Status
RUO
Other Names
Granulocyte/macrophage-colony stimulating factor, CSF-α, Pluripoietin-α, Eosinophil colony stimulating factor (Eo-CSF), Burst promoting activity (BPA)
Isotype
Rat IgG2a, κ
MP1-22E9_PerCPCyanine55_GM-CSF_Antibody_1_120623
PMA+ Ionomycin-stimulated Th2-polarized C57BL/6 splenocytes were surface stained with CD3 FITC and intracellularly stained with GM-CSF (clone MP1-22E9) PerCP/Cyanine5.5 (left) or rat IgG2a, κ PerCP/Cyanine5.5 isotype control (right).
  • MP1-22E9_PerCPCyanine55_GM-CSF_Antibody_1_120623
    PMA+ Ionomycin-stimulated Th2-polarized C57BL/6 splenocytes were surface stained with CD3 FITC and intracellularly stained with GM-CSF (clone MP1-22E9) PerCP/Cyanine5.5 (left) or rat IgG2a, κ PerCP/Cyanine5.5 isotype control (right).
Compare all formats See PerCP/Cyanine5.5 spectral data
Cat # Size Price Quantity Check Availability
505409 25 µg $141.00
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505410 100 µg $352.00
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Description

GM-CSF is a hematopoietic factor that is produced by T cells, macrophages, fibroblasts and endothelial cells. This multifunctional cytokine stimulates progenitor cells of neutrophils, eosinophils, and macrophages. GM-CSF is also a differentiation and activating factor for granulocytic and monocytic cells.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Yeast-expressed, recombinant mouse GM-CSF
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

ELISA or ELISPOT Capture1,3-5: The purified MP1-22E9 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated MP1-31G6 antibody (Cat. No. 505502) as the detecting antibody. The LEAF™ purified antibody is suggested for ELISPOT capture.
Flow Cytometry8: The fluorochrome-labeled MP1-22E9 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify GM-CSF -producing cells within mixed cell populations.
Neutralization2-4: The MP1-22E9 antibody can neutralize the bioactivity of natural or recombinant GM-CSF. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for in vivo and in vitro neutralization (Cat. No. 505408).
Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraformaldehyde-fixed, saponin-treated frozen tissue sections1,6,7, and immunocytochemistry8.

Application References

(PubMed link indicates BioLegend citation)
  1. Sander B, et al. 1993. J. Immunol. Methods 166:201.
  2. Suda T, et al. 1990. Cell. Immunol. 129:228.
  3. Nozaki S, et al. 1991. J. Invest. Dermatol. 97:10.
  4. Abrams JS, et al. 1992. Immunol. Rev. 127:5.
  5. Abrams JS. 2001. Curr. Protoc. Immunol. Unit 6.20.
  6. Sander B, et al. 1991. Immunol. Rev. 119:65.
  7. Andersson U, et al. 1999. Detection and quantification of gene expression. New York:Springer-Verlag.
  8. Larkin J, et al. 2006. J. Immunol. 177:268.
Product Citations
  1. Zhou L, et al. 2022. PLoS Negl Trop Dis. 16:e0010851. PubMed
  2. Dikiy S, et al. 2021. Immunity. 54(5):931-946.e11. PubMed
  3. Gurusamy M, et al. 2021. Nat Commun. 12:6798. PubMed
RRID
AB_2562376 (BioLegend Cat. No. 505409)
AB_2562376 (BioLegend Cat. No. 505410)

Antigen Details

Structure
Cytokine; 22 kD (Mammalian)
Bioactivity
Growth/development granulocyte/macrophage progenitors; differentiates myeloblasts/monoblasts; synergizes with Epo proliferation of erythroid/megakaryocytic progenitors
Cell Sources
T cells, monocytes/macrophages, fibroblasts, endothelial cells, mast cells
Cell Targets
Granulocyte/macrophage/erythroid/megakaryocytic progenitors, myeloblasts, monoblasts
Receptors
Heterodimer GM-CSFR α subunit (CDw116); β-subunit (CDw131) in common with IL-3R, IL-5R
Biology Area
Cell Biology, Stem Cells
Molecular Family
Cytokines/Chemokines, Growth Factors
Antigen References

1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Demetri, G., et al. 1991. Blood 78:2791.
3. Fan, D., et al. 1991. In vivo 5:571.
4. Negrin, R., et al. 1992. Adv. Pharmacol. 23:263.

Regulation
Synergistic with IL-1, IL-3, G-CSF; E21R competitive antagonist for receptor binding; stored in ECM with heparan sulfate proteoglycans
Gene ID
12981 View all products for this Gene ID
UniProt
View information about GM-CSF on UniProt.org

Other Formats

View All GM-CSF Reagents Request Custom Conjugation
Description Clone Applications
FITC anti-mouse GM-CSF MP1-22E9 ICFC
PE anti-mouse GM-CSF MP1-22E9 ICFC
Purified anti-mouse GM-CSF MP1-22E9 ELISA Capture,ELISPOT Capture,Neut,ICC,IHC-F
PerCP/Cyanine5.5 anti-mouse GM-CSF MP1-22E9 ICFC
APC anti-mouse GM-CSF MP1-22E9 ICFC
PE/Cyanine7 anti-mouse GM-CSF MP1-22E9 ICFC
Ultra-LEAF™ Purified anti-mouse GM-CSF MP1-22E9 ELISA Capture,ELISPOT Capture,Neut,ICC,IHC-F
PE/Dazzle™ 594 anti-mouse GM-CSF MP1-22E9 ICFC
APC/Fire™ 750 anti-mouse GM-CSF MP1-22E9 ICFC
Pacific Blue™ anti-mouse GM-CSF MP1-22E9 ICFC
Brilliant Violet 421™ anti-mouse GM-CSF MP1-22E9 ICFC
Go To Top Version: 1    Revision Date: 08/15/2013

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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