- Clone
- MA-69 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Igh-6a
- Isotype
- Mouse (C57BL/6) IgG1, κ
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BALB/c mouse spenocytes were stained with PerCP/Cyanine5.5 IgMa (clone MA-69, closed histogram) or PerCP/Cyanine5.5 mouse IgG1, κ isotype control (open histogram).
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 408611 | 25 µg | $147.00 | |||
| 408612 | 100 µg | $323.00 | |||
This antibody was raised by immunization with IgMa mAb TC31. It specifically reacts with mouse IgM of Igh-Ca and the related haplotypes (c, f, g, h, and j). It has been reported that beads coated with MA-69 antibody can stimulate B cell proliferation.
Product Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
- Excitation Laser
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Blue Laser (488 nm)
- Application Notes
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Additional reported applications (for the relevant formats) include: ELISA as capture antibody or detection antibody, stimulation of B cell proliferation, and immunohistochemistry of acetone-fixed frozen sections.
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Application References
(PubMed link indicates BioLegend citation) -
- Sieckmann D, et al. 1991. Hybridoma 10:121.
- Stall AM. 1996. Weir's Handbook of Experimental Immunology pp. 27.
- RRID
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AB_2728435 (BioLegend Cat. No. 408611)
AB_2728435 (BioLegend Cat. No. 408612)
Other Formats
View All IgMa Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Biotin anti-mouse IgMa | MA-69 | ELISA Detection,FC,IHC |
| FITC anti-mouse IgMa | MA-69 | FC |
| PE anti-mouse IgMa | MA-69 | FC |
| Purified anti-mouse IgMa | MA-69 | FC,IHC,ELISA |
| PerCP/Cyanine5.5 anti-mouse IgMa | MA-69 | FC |
| APC anti-mouse IgMa | MA-69 | FC |
| PE/Cyanine7 anti-mouse IgMa | MA-69 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Biotin anti-mouse IgMa
BALB/c splenocytes stained with MA-69 Biotin, followed by Sa... -
FITC anti-mouse IgMa
Balb/c (upper) and C57BL/6 (bottom) splenocytes stained with... -
PE anti-mouse IgMa
Balb/c mouse splenocytes stained with MA-69 PE -
Purified anti-mouse IgMa
BALB/c mouse splenocytes stained with purified MA-69, follow... -
PerCP/Cyanine5.5 anti-mouse IgMa
BALB/c mouse spenocytes were stained with PerCP/Cyanine5.5 I... -
APC anti-mouse IgMa
BALB/c mouse spenocytes were stained with APC IgMa (clone MA... -
PE/Cyanine7 anti-mouse IgMa
BALB/c mouse spenocytes were stained with PE/Cyanine7 IgM