PerCP/Cyanine5.5 Streptavidin

Product Details

Verified Reactivity

Human, Mouse, Rat, All Species

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

Streptavidin is conjugated with PerCP/Cyanine5.5 under optimal conditions.

Concentration

0.2 mg/ml (concentration relates to the Streptavidin only component of the conjugate)

Storage & Handling

The Streptavidin-PerCP/Cyanine5.5 solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

ICFC - Verified

Recommended Usage

Each lot of this Streptavidin-PerCP/Cyanine5.5 is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the PerCP/Cyanine5.5 conjugation. For immunofluorescent staining, the recommended use of this reagent is = 0.06 µg per 10⁶ cells in 100 µl staining volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

Streptavidin-PerCP/Cyanine5.5 is useful as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. The average molecular weight of Streptavidin-PerCP/Cyanine5.5 is 110 kD and Streptavidin alone is 52 kD.

Application References

(PubMed link indicates BioLegend citation)

1. Killeen ME, et al. 2013. J. Immunol. 190:4324. PubMed
2. Grage-Griebenow E, et al. 2014. Mol Oncol. 8:982. PubMed
3. Mise-Omata S, et al. 2014. Int Immunol. 26:607. PubMed

Product Citations

1. Suzuki M, et al. 2015. J Immunol. 195: 1273-1281. PubMed
2. Li X, et al. 2022. Haematologica. 107:2576. PubMed
3. Koizumi M, et al. 2022. J Biol Chem. 298:102506. PubMed
4. Tanaka T, et al. 2023. Nat Immunol. 24:439. PubMed
5. Feng X, et al. 2023. Nat Commun. 14:3208. PubMed
6. Grage-Griebenow E, et al. 2014. Mol Oncol. 8:982. PubMed
7. Zakeri A, et al. 2021. J Extracell Vesicles. 10:e12131. PubMed
8. Hirano KI, et al. 2021. eLife. 0.416666666666667. PubMed
9. Pease NA, et al. 2021. Cell Reports. 34(12):108888. PubMed
10. Greenblatt SM, et al. 2018. Cancer Cell. 33:1111. PubMed
11. Ng KK et al. 2018. eLife. 7 pii: e37851. PubMed
12. Perelman SS, et al. 2021. Nat Microbiol. 6:731. PubMed
13. Dong S, et al. 2021. Nature. 591:117. PubMed
14. Glaros V, et al. 2021. Immunity. 54:2005. PubMed
15. Wittner J, et al. 2022. Mucosal Immunol. 15:668. PubMed
16. Fournier M, Bijl C 2015. Stem Cells Dev. 24: 2413-2422. PubMed
17. Delacher M, et al. 2020. Immunity. 52:295. PubMed
18. Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed
19. Fujii T, et al. 2021. Bone Res. 9:4. PubMed
20. Verheijen M, et al. 2020. Cell Rep. 108376:33. PubMed
21. Yuan X, et al. 2017. Elife. 6:e29540. PubMed
22. Pi J, et al. 2022. J Nanobiotechnology. 20:36. PubMed
23. Mise-Omata S, et al. 2014. Int Immunol. 26:607. PubMed
24. Lopes N, et al. 2022. Elife. 11:. PubMed
25. Wagner AK, et al. 2022. iScience. 25:105137. PubMed
26. Yoshinaga M, et al. 2022. Nat Commun. 13:6435. PubMed
27. Killeen M, et al. 2013. J Immunol. 190:4324. PubMed
28. Li X, et al. 2019. Stem Cells. 37:937. PubMed
29. Boguslawski KM, et al. 2020. Sci Adv. 6:eaax7515. PubMed
30. Furusawa J, et al. 2016. PLoS Pathog. 12: 1005507. PubMed

RRID

AB_2716577 (BioLegend Cat. No. 405214)

Antigen Details

Gene ID

NA

UniProt

View information about Biotin on UniProt.org

Related Protocols

• Cell Surface Flow Cytometry Staining Protocol