- Clone
- 1D12 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- HCDM listed
- Other Names
- T cell immunoglobulin mucin 1, TIMD-1, KIM-1 (kidney injury molecule-1), HAVCR-1, CD365
- Isotype
- Mouse IgG1, κ
-
769-P, human kidney cell line was stained with anti-human CD365 (Tim-1) (clone 1D12) PerCP/Cyanine5.5 (filled histogram) or mouse IgG1, κ PerCP/Cyanine5.5 isotype control (open histogram).
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 353911 | 25 tests | $183.00 | |||
| 353912 | 100 tests | $417.00 | |||
CD365 (Tim-1) is a transmembrane glycoprotein expressed on activated CD4+ lymphocytes especially on Th2 cells. It has been implicated to play a critical role in the development of atopic disease and other Th2-biased immune responses. Tim-1 is a hepatitis A virus receptor in humans. Tim-4 is the endogenous ligand of Tim-1. The interaction of Tim-1 and Tim-4 is involved in the costimulation of T cell proliferation. Tim-1 has been reported to be an endogenous ligand for LMIR5 (Leukocyte mono-immunoglobulin (Ig)-like receptor 5), and the Tim-1-LMIR5 interaction plays a physiological role in immune regulation by myeloid cells.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human TIM-1-IgV Fc
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm. - Excitation Laser
-
Blue Laser (488 nm)
- Application Notes
-
Additional reported application (for the relevant formats) include: blocking function1.
-
Application References
(PubMed link indicates BioLegend citation) -
- Freeman GJ, et al. 2007. Immunity. 27:927. (Block)
- RRID
-
AB_2860929 (BioLegend Cat. No. 353911)
AB_2860929 (BioLegend Cat. No. 353912)
Antigen Details
- Structure
- Type 1 transmembrane glycoprotein.
- Distribution
-
Activated CD4+ lymphocytes, especially on Th2 cells.
- Function
- TIM-1 ligation induces T cell proliferation, promotes cytokine production, and aids in the regulation of Th2 immunity.
- Ligand/Receptor
- TIM-4
- Cell Type
- Dendritic cells, T cells, Th2
- Biology Area
- Immunology, Inhibitory Molecules
- Molecular Family
- CD Molecules
- Antigen References
-
1. Freeman GJ, et al. 2007. Immunity. 27:927.
2. Meyers JH, et al. 2005. Nat. Immunol. 6:455.
3. Kuchroo VK, et al. 2003. Nat. Rev. Immunol. 3:454.
4. Mariat C, et al. 2005. Philos. Trans. R. Soc. Lond. B. 360:1681.
5. Yamanishi Y, et al. 2010. J. Exp. Med. 207:1501. - Gene ID
- 26762 View all products for this Gene ID
- UniProt
- View information about CD365 on UniProt.org
Other Formats
View All CD365 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-human CD365 (Tim-1) | 1D12 | FC |
| PE anti-human CD365 (Tim-1) | 1D12 | FC |
| APC anti-human CD365 (Tim-1) | 1D12 | FC |
| PerCP/Cyanine5.5 anti-human CD365 (Tim-1) | 1D12 | FC |
| PE/Cyanine7 anti-human CD365 (Tim-1) | 1D12 | FC |
| TotalSeq™-C0869 anti-human CD365 (Tim-1) Antibody | 1D12 | PG |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Purified anti-human CD365 (Tim-1)
769-P, human kidney cell line was stained with purified anti... -
PE anti-human CD365 (Tim-1)
769-P, human kidney cell line was stained with anti-human CD... -
APC anti-human CD365 (Tim-1)
Human kidney cell line 769-P was stained with anti-human CD3... -
PerCP/Cyanine5.5 anti-human CD365 (Tim-1)
769-P, human kidney cell line was stained with anti-human CD... -
PE/Cyanine7 anti-human CD365 (Tim-1)
769-P, human kidney cell line was stained with anti-human CD... -
TotalSeq™-C0869 anti-human CD365 (Tim-1) Antibody