- Regulatory Status
- RUO
- Other Names
- Counting Beads
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Human peripheral blood lymphocytes were stained with anti-human CD3 FITC/CD4 PE/CD45 PerCP Cocktail using a lyse, no-wash method. Precision Count Beads™ were added and bead count and CD4+CD3+ cell count was determined by gating on beads and cells as shown, gated on CD45+ lymphocytes. The number of total cells is determined as described in the protocol. -
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 424902 | 100 tests | $258.00 | |||
Precision Count Beads™ can be used to obtain absolute counts of cells or any other samples acquired on a flow cytometer. Precision Count Beads™ are fluorescent particles about 10µm that can be detected across a broad range of wavelengths (405-635nm excitation and 400-800nm emission). Absolute numbers of cells can be determined by comparing the bead count and cell count. Precision Count Beads™ can work with all kinds of assays including a lyse, no-wash whole blood assay.
Product Details
- Formulation
- Precision Count Beads™ are supplied in a solution containing 0.02% sodium azide and 0.01% NP40.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- Store between 2°C and 8°C.
- Application
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FC - Quality tested
- Recommended Usage
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Vigorously vortex the Precision Count Beads™ bottle for 30-40 seconds to ensure complete mixing and break up of aggregates that may occur during storage. For flow cytometric analysis, the suggested use of this reagent is 100 µl per test. Please read the Precision Count Beads™ protocol and applications for further instructions.
- Application Notes
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Precision Count Beads™ are designed for counting the absolute number of cells in a complex population and other particles by flow cytometry. Precision Count Beads™ are excited by a variety of lasers including violet (405nm), blue (488nm), yellow/green (562nm), and red (633nm).
- Product Citations
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Antigen Details
- Gene ID
- NA