- Regulatory Status
- RUO
- Other Names
- protein ladder, protein marker, pre-stained protein ladder, pre-stained protein marker, protein molecular weight marker
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3µl Prime-Step™ Prestained Broad Range Protein Ladder was resolved by electrophoresis in different gel running systems, including 14%, 4-20% and 4-12% Tris-Glycine gel, 4-12% Bis-Tris gel (MOPS and MES running buffer), 3-8% Tris-Acetate gel and 10-20% Tricine gel. Then protein ladders were transferred to nitrocellulose membrane. Mw: molecular weight. -
3µl Prime-Step™ Prestained Broad Range Protein Ladder (lane M) and total cell lysates (15µg total protein) was resolved by electrophoresis in 4-12% Bis-Tris gel (MOPS running buffer). Then protein ladders and total cell lysates were transferred to nitrocellulose (NC) and PVDF membrane followed by Ponceau red staining. Lane 1: Hela; Lane 2: Jurkat; Lane 3: NIH3T3 cells. -
3µl Prime-Step™ Prestained Broad Range Protein Ladder (lane M) and 2µg recombinant proteins 1, 2, 3, with different molecular weights, was resolved by electrophoresis in 12% Bis-Tris gel (MES running buffer) followed by Coomassie blue staining. -
3µl Prime-Step™ Prestained Broad Range Protein Ladder was resolved by electrophoresis in different gel running systems: 4-20% Tris-Glycine gel, 4-12% Bis-Tris gel (MES running buffer). Proteins were either stained with Coomassie blue, or transferred to nitrocellulose membrane followed by Ponceau red staining . Lane 1: Prime-Step™ Prestained Broad Range Protein Ladder; Lane 2: Competitor 1 protein ladder; Lane 3: Competitor 2 protein ladder.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 773301 | 50 µL | $36.00 | |||
| 773302 | 500 µL | $124.00 | |||
The Prime-Step™ Prestained Broad Range Protein Ladder is a three-color protein standard with 10 pre-stained proteins covering a wide range of molecular weights from 6.5 to 270 kDa. It has 7 blue-stained bands and 3 reference bands (2 orange bands at 30 kDa and 270 kDa and 1 green band at 52 kDa) when separated on Tris-glycine gel. The Prime-Step™ Prestained Broad Range Protein Ladder is designed for monitoring protein separation during SDS-PAGE electrophoresis, confirming Western blot transfer quality on membranes (PVDF or nitrocellulose membrane) and for approximating the size of target proteins on the gel or blot.
Key Product Features
- Clear separation and sharp bands in different SDS-PAGE gel and buffer systems
- Excellent transfer efficiency on PVDF and Nitrocellulose membrane for WB
- Suitable for size detection during protein purification
- Comparable MW detection with competitor’s protein ladders
- Better performance for high molecular weight proteins
- Supplied in the sample loading buffer and is ready to use
Product Details
- Formulation
- Sample loading buffer (62.5 mM Tris-H3PO4 (pH 7.5 at 25°C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN3, 33% glycerol).
- Preparation
- Chromophore conjugated recombinant protein mixture supplied in sample loading buffer.
- Storage & Handling
- Upon receipt, store at -20°C.
- Application
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WB - Quality tested
- Recommended Usage
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• 3-5 μl per loading for clear visualization during electrophoresis on 15-well mini-gel or 20-well midi-gel, respectively.
• 3-5 μl per well for general Western transferring.
The ladder is supplied in the sample loading buffer and is ready to use. - Application Notes
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The apparent molecular weight (kDa) of each protein has been determined by calibration against unstained protein standards.
Antigen Details
- Biology Area
- Cell Biology
- Gene ID
- NA