Purified anti-Acetylated Lysine Antibody

Pricing & Availability
Clone
15G10 (See other available formats)
Regulatory Status
RUO
Other Names
Proteins are acetylated by the reversible transfer of acetyl-CoA to the ε-amino group of lysine
Isotype
Mouse IgG2b, κ
15G10_PURE_AcetylatedLysine_Antibody_1_WB_051016
Western blot analysis of HeLa untreated (lane 1) and HeLa starved overnight and treated with sodium butyrate overnight (lane 2) using anti-Acetylated Lysine (clone 15G10) antibody. Purified anti-GAPDH (clone Poly6314) antibody was used as a loading control.
  • 15G10_PURE_AcetylatedLysine_Antibody_1_WB_051016
    Western blot analysis of HeLa untreated (lane 1) and HeLa starved overnight and treated with sodium butyrate overnight (lane 2) using anti-Acetylated Lysine (clone 15G10) antibody. Purified anti-GAPDH (clone Poly6314) antibody was used as a loading control.
  • 15G10_PURE_AcetylatedLysine_Antibody_2_IF_051016
    Untreated HeLa cells (upper panel), or overnight nocodazole treated HeLa cells (lower panel) stained with purified mouse monoclonal antibody against Acetylated Lysine (clone 15G10), followed by Rhodamine Red-X conjugated Donkey anti-mouse IgG and DAPI.
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Cat # Size Price Quantity Check Availability
623401 25 µg $101.00
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623402 100 µg $218.00
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Description

Proteins are reversibly and dynamically acetylated on the ε-amino group of lysine by acetyltransferases and deacetylated by deacetylases. This post-translational modification can regulate protein function (interactions with other proteins and DNA binding). Histones and transcription factors (PCAF, p53, p300, etc) appear to be the major targets of acetyltransferases. Acetylation is usually associated with chromatin remodeling and transcriptional activation, although in some cases (telomeres) it is associated with gene silencing. The 15G10 antibody recognizes acetylated lysine residues on proteins.

Technical data sheet

Product Details

Verified Reactivity
Species independent
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Acetylated protein mixture
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, store between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 µg antibody per 5 ml antibody dilution buffer for each mini-gel. For immunocytochemistry: Use a dilution range of 1-4 µg/ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Kuo HP, et al. 2010. Sci Signal. 3:Ra9. PubMed
Product Citations
  1. Yang JM, et al. 2022. Sci Adv. 8:eabo0183. PubMed
  2. Zhang W, et al. 2022. J Clin Invest. 132: . PubMed
  3. Sun X, et al. 2022. J Am Heart Assoc. 11:e025266. PubMed
  4. Lee J, Homma T, Fujii J, et al. 2017. Toxicol Lett. 10.1016/j.toxlet.2017.06.005. PubMed
  5. Wu Q, et al. 2021. J Immunother Cancer. 9: . PubMed
  6. Kuo H, et al. 2010. Sci Signal. 3:ra9. PubMed
  7. Chen G, et al. 2019. EMBO Mol Med. 11:e10469. PubMed
  8. Han S, et al. 2017. Nat Commun. 8:14568. PubMed
RRID
AB_315967 (BioLegend Cat. No. 623401)
AB_315967 (BioLegend Cat. No. 623402)

Antigen Details

Structure
Proteins are acetylated by the reversible transfer of acetyl-CoA to the ε-amino group of lysine
Function
Post-translational modification of proteins by acetyltransferases and deacetylated by deacetylases to regulate protein-protein and protein-DNA interactions
Biology Area
Cell Biology, Chromatin Remodeling/Epigenetics
Antigen References

1. Wang S, et al. 2004. Novartis Found. Symp. 259:238.
2. Legube G, et al. 2003. EMBO Rep. 4:944.
3. Chen LF, et al. 2003. J. Mol. Med. 81:549.

Gene ID
8850 View all products for this Gene ID
UniProt
View information about Acetylated Lysine on UniProt.org
Go To Top Version: 3    Revision Date: 09/19/2023

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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