- Clone
- Poly28635 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- ADH1, Alcohol Dehydrogenase 1C (Class I), Gamma Polypeptide, Alcohol Dehydrogenase Subunit Gamma, Aldehyde Reductase, EC 1.1.1
- Isotype
- Rabbit Polyclonal IgG
-
Western blot of purified anti-ADH1 antibody (Poly28635). Lane 1: Molecular weight marker; Lane 2: 20 µg of human liver lysate; Lane 3: 5 µg of mouse liver lysate; Lane 4: 10 µg of rat liver lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP Donkey anti-rabbit IgG Antibody (Cat. No. 406401). Enhanced chemiluminescence was used as the detection system. -
IHC staining of purified anti-ADH1 antibody (Poly28635) on formalin-fixed paraffin-embedded human liver tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm -
IHC staining of purified anti-ADH1 antibody (Poly28635) on formalin-fixed paraffin-embedded mouse liver tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm -
IHC staining of purified anti-ADH1 antibody (Poly28635) on formalin-fixed paraffin-embedded rat liver tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 863501 | 25 µg | $125.00 | |||
ADH1 is the γ subunit of class I alcohol dehydrogenase. The alcohol dedydrogenase family is involved in metabolization of a variety of substrates, including ethanol and lipid peroxidation products. ADH1 is related to the signaling pathway of cytochrome P450 metabolism. It is also associated with several diseases including late-onset Parkinson Disease.
Product Details
- Verified Reactivity
- Human, Mouse, Rat
- Antibody Type
- Polyclonal
- Host Species
- Rabbit
- Immunogen
- Recombinant Alcohol Dehydrogenase 1 (ADH1) expressed in E.coli.
- Formulation
- 0.01 M PBS, pH 7.4, containing 0.02% NaN3, 50% glycerol.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- Store at 4°C for up to 3 months. For longer term storage, aliquot into small volumes and store at -20°C.
- Application
-
WB - Quality tested
IHC-P - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 - 1.0 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_2820148 (BioLegend Cat. No. 863501)
Antigen Details
- Structure
- ADH1 is a 375 amino acid protein with an apparent molecular mass of ~39 kD.
- Distribution
-
Tissue Distribution: Liver, kidney, pancreas, and gallbladder
Cellular Distribution: Cytosol and nucleus - Function
- ADH1 is involved in ethanol and hydroxysteroids metabolism.
- Interaction
- Ethanol, retinol, hydroxysteroids
- Cell Type
- Mesenchymal cells
- Biology Area
- Cell Biology, Neurodegeneration, Neuroscience
- Molecular Family
- Aldehyde Dehydrogenases
- Antigen References
-
- Lieber CS. 2004. Alcohol. 34(1):9-19.
- Zuo L, et al. 2013. Hum Genet. 132(7):735-43.
- Dumont S, et al. 2018. PLoS One. 13(9):e0204530.
- Gene ID
- 126 View all products for this Gene ID 11522 View all products for this Gene ID 24172 View all products for this Gene ID
- UniProt
- View information about ADH1 on UniProt.org
Other Formats
View All ADH1 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-ADH1 | Poly28635 | WB,IHC-P |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
